The cellular kinetics of repair of BrdU-tagged HSPCs were different every 12 h between day 3 and day 4. Moreover, HSPCs still retained labels and located definitively in the periportal region after a prolonged chase. CONCLUSIONS: The LRC method together with our novel KAL method reported here ...
Cell features describing morphology, granularity, biomass, etc are extracted from the images. (d) These biophysical features are used in a machine learning algorithm for high-accuracy label-free classification of the cells. (Fig. 1). Each pulse representing one frame of the camera is then ...
Type-2 diabetes mellitus (T2DM) therapy requires early diagnosis and complication avoidance. Unfortunately, current diagnostic markers do not meet these needs. Data-independent acquisition mass spectrometry (DIA-MS) offers a solution for clinical diagnos
Furthermore, the comparison also indicates a region (right lower quarter in image a), where signals of the SERS labels exist, but no lipids are present there. On the other hand, the right lower corner of image b indicates a region where lipid signals exist but the SERS signal of the ...
Collecting and analysing the IR light backscattered from the small region where the tip and sample interact allows us to infer the IR absorption properties of the sample material in that region. Tuning the laser allows us to image with chemical specificity without the need for staining or ...
All Zorya proteins preferentially interact with the CL-rich polar regions of the inner membrane. In addition, ZorA likely has a weak interaction with the equatorial region of the inner membrane and ZorE likely has a reversible interaction with the equatorial region of the inner membrane. Full ...
This allows for the peptide strand to pass through the nanopore constriction with a regular stepping size and yields sequence-dependent signals derived from the peptide strand in the pore's sensitive region. Oligonucleotide-assisted translocation In nanopore-based DNA and RNA sensing, nucleic acid ...
b Schematic detailing our automated image analysis pipeline for deter- mining the distribution of target proteins between the Golgi (GALNT2 region) and the rest of the cell (phalloidin region). A decrease in the corresponding ratio indi- cates that a target protein is moving out of the Golgi ...
The function of the ion processor is similar to the combination of a C-Trap and IRM, albeit operating much faster. Admitted ions are accumulated and fragmented via higher-energy collisional dissociation (HCD)16 in a high-pressure region, and then passed at low energy to a low-pressure region...
2b). As expected, the correlation from the same sub-region replicates is higher than that from different sub-region replicates (Fig. 2b). This further confirmed high reproducibility of SOP-MS for processing small numbers of cells. Fig. 2: SOP-MS analysis of LCM-dissected mouse uterine tissue...