Identification of label-retaining cells in mouse endometrium. Human and mouse endometrium (lining of the uterus) undergo cycles of growth and regression as part of each reproductive cycle. A well-known method to ident... RWS Chan,CE Gargett - 《Stem Cells》 被引量: 794发表: 2010年 ...
This technique applies two polarized light beams perpendicular to each other, to detect edges of cellular components and converts them either in intensity or color difference with high lateral and axial resolution, giving a pseudo-3D appearance of the image. Fluorescence Microscopy Another solution ...
A short-lived dip in frequency is systematically produced at the beginning of each step. This is due to the introduction of a different solution into the QCMD modules (Fig. 2a). This short-lived dip in frequency, also present when a TXTL reaction is introduced into the QCMD chambers, is ...
Type-2 diabetes mellitus (T2DM) therapy requires early diagnosis and complication avoidance. Unfortunately, current diagnostic markers do not meet these needs. Data-independent acquisition mass spectrometry (DIA-MS) offers a solution for clinical diagnos
but most of the existing methods have the following two limitations: (1) their coverage scope is less than ten locations and hence many organelles in an eukaryotic cell cannot be covered, and (2) they can only be used to deal with single-label systems in which each of the constituent prot...
The first complete proteome of a eukaryotic organism analyzed by MS was that of yeast22, and with state-of-the-art instrumentation it was possible to cover ~4,000 yeast proteins in approximately 1 h of LC–MS/MS time23. Analyzing whole yeast cell digests with nDIA (2-Th) across ...
Next we described the ensemble voting strategies of our method. Basic classifiers are denoted asC = {ci : i = 1, 2, ..., p}, and the labels are denoted asY = {λ j : j = 1, 2, ..., q}. MeanEnsemble algorithm. calculate the average value The prediction of each column. ...
each accession number may be associated with zero, one or more distinct GO terms. Conversely, one GO term may be associated with zero, one, or many different accession numbers. This means that the mappings between accession numbers and GO terms are many-to-many....
DIA maps outperform DDA maps across all metrics First, we extensively optimized DIA-methods for generating label-free DIA-DOMs using a benchmark sample set prepared from three inde- pendent subcellular fractionations from HeLa cells, each with six fractions, label-free, as described20. We tested ...
(and thus dry mass) that correlate to patterns of cellular and sub-cellular dynamics within the sample. Specifically, in DqOBM, a sample is imaged over a period of time, and the resulting temporal signal for each spatial pixel is analyzed based on its temporal frequency response, which ...