Identification of label-retaining cells in mouse endometrium. Human and mouse endometrium (lining of the uterus) undergo cycles of growth and regression as part of each reproductive cycle. A well-known method to ident... RWS Chan,CE Gargett - 《Stem Cells》 被引量: 794发表: 2010年 ...
Table 1 Demographic and clinical characteristics of patients with T2DM and healthy control (HC). Full size table 10–12 h following an overnight fast, a venous blood sample was collected from each participant, placed in plain tubes, centrifuged at 3000 × g for 10 min, and the serum...
This technique applies two polarized light beams perpendicular to each other, to detect edges of cellular components and converts them either in intensity or color difference with high lateral and axial resolution, giving a pseudo-3D appearance of the image. Fluorescence Microscopy Another solution ...
A short-lived dip in frequency is systematically produced at the beginning of each step. This is due to the introduction of a different solution into the QCMD modules (Fig. 2a). This short-lived dip in frequency, also present when a TXTL reaction is introduced into the QCMD chambers, is...
The technical differences and individual strengths of each method are extensively reviewed elsewhere4. The DOMs approach is particularly powerful for comparative applications, due to its robust fractionation protocol. Briefly, cells are lysed mechanically and the released organelles are partially separated ...
but most of the existing methods have the following two limitations: (1) their coverage scope is less than ten locations and hence many organelles in an eukaryotic cell cannot be covered, and (2) they can only be used to deal with single-label systems in which each of the constituent prot...
Each MS acquisition batch had >10 QC samples allowing to correct for the most evident batch effects attributed to an acquisition date (Suppl. Fig. 12). Signal correction was performed using ComBat approach36 by specifying covariates that includes QC samples and genotypes of measured mutants using ...
annotations and UniProtKB database can be exploited for subcellular localization. Specifically, given the accession number of a protein, a set of GO terms can be retrieved from the GO annotation database filec. In UniProKB, each protein has an accession number, and in the GO annotation data...
a sample is imaged over a period of time, and the resulting temporal signal for each spatial pixel is analyzed based on its temporal frequency response, which exhibits an exponential-like behavior that can be characterized using phasor analysis, as illustrated in Fig.1(more details in the “Res...
The 10 bulls in each of the R1 and A1 treatment groups were slaughtered at the end of the 125 day ‘Period 1’. At the end of the 55 day ‘Period 2’ all animals in the remaining two treatment groups; R2 and A2 were slaughtered. At both slaughter time-points, the slaughter order ...