L929成纤维细胞瘤细胞(l929-a)和L929纤维肉瘤细胞(l929-n)是不同的细胞系,常用于肿瘤坏死的细胞毒性因子α(TNFα)的研究,TNFa已被报道可诱导两种细胞系的坏死。然而,当比较TNFα诱导的在这两个细胞系的细胞死亡时,l929-n表现出典型的RIP3依赖性细胞坏死,在l929-a中却不是。现分享一篇运用siRNA转染(Entranster...
A. CossarizzaTaylor & FrancisFree Radical ResearchPolla B,Jacquier-Sarlin M,et al.TNF alpha alters mitochondrial membrane potential in L929 but not in TNF alpha-resistant L929.12 cells: relationship with the expression of stress proteins, annexin 1 and superoxide dismutase activity.Free Radical ...
L929 is a murine aneuploid fibrosarcoma cell line which isoften used to assay TNF-alpha and TNF-beta. Treatment with TNF initiatesapoptosis and subsequent cell death, therefore L929 cell is often used forimmunological assays. However L929 cell is hard to transfect, especiallyresistant to...
PCR方法观察SPRY2基因在不同质量浓度TNF-α刺激作用下表达水平的变化;应用重组腺病毒介导SPRY2基因过表达方法观察其对TNF-α细胞毒活性的调解效应.结果 L929细胞中存在SPRY2基因的天然表达,并且其表达水平随TNF-α刺激质量浓度的升高而升高,过表达SPRY2基因促进TNF-α诱导的L929细胞死亡.结论 SPRY2分子在TNF-α介导...
We confirmed with the use of crystal violet bioassay the high susceptibility of mouse L929 cells to the cytotoxic action of recombinant tumor necrosis factor-alpha (TNF). However, when a [3H]thymidine release assay was used for the same purpose, we found that [3H]thymidine uptake by the ...
机译:肿瘤坏死因子-α(TNF-α)在类风湿关节炎的发病机理中起着重要作用。本研究旨在评估脂多糖(LPS),植物分裂素和细胞分化剂对人黏附单核细胞(AMC)分泌的TNF-α的细胞毒性的影响。通过L929生物测定系统分析了LPS处理,植物分裂素处理和细胞分化剂处理的AMC上清液中TNF-α的细胞毒性。我们的研究结果表明,LPS可以诱导...
Hepatology Volume 22, Issue 4, Part 2, October 1995, Pages A485AASLD AbstractTumor necrosis factor alpha (TNF) increases ATP content in metabolically inhibited HepG2 and L929 cells preceding cell death: Department of Medical Research. Unit of Digestive Diseases, Hospital “12 de Octubre”, “...
Interleukin-1β (IL-1β) is a cytokine that shares with tumor necrosis factor (TNF) the ability to initiate largely similar signaling pathways, leading to proinflammatory gene expression. In contrast to TNF, however, IL-1β is not believed to induce tumor cell death. Here we demonstrate that...
L929成纤维细胞瘤细胞(l929-a)和L929纤维肉瘤细胞(l929-n)是不同的细胞系,常用于肿瘤坏死的细胞毒性因子α(TNFα)的研究,TNFa已被报道可诱导两种细胞系的坏死。然而,当比较TNFα诱导的在这两个细胞系的细胞死亡时,l929-n表现出典型的RIP3依赖性细胞坏死,在l929-a中却不是。现分享一篇运用siRNA转染(Entranster...
L929成纤维细胞瘤细胞(l929-a)和L929细胞(l929-n)是不同的细胞系,常用于肿瘤坏死的细胞毒性因子α(TNFα)的研究,TNFa已被报道可诱导两种细胞系的坏死。然而,当比较TNFα诱导的在这两个细胞系的细胞死亡时,l929-n表现出典型的RIP3依赖性细胞坏死,在l929-a中却不是。现分享一篇运用...