L_RNA_scaffolderScaffoldingTranscriptomeBackground: Generation of large mate-pair libraries is necessary for de novo genome assembly but the procedure is complex and time-consuming. Furthermore, in some complex genomes, it is hard to increase the N50 length even with large mate-pair libraries, ...
L_RNA_scaffolder能利用长的转录子序列来辅助基因组的scaffolding拼接。这些长的转录子序列可以是454/sanger/Ion_Torrent的测序结果,也可以是illumina测序的de novo组装结果。 在使用此软件进行scaffolding之前,需要将这些长的转录子序列使用BLAT软件map到基因组上,得到PSL(without heading)文件。将此PSL文件和基因组的初...