In order to enable real-time monitoring of orthotopically engrafted tumor growth using optical imaging, we generated and characterized a firefly luciferase (Luc) expressing model via lentivirus transfection of the syngeneic cell line derived from parental KPC MuPrime tumors.Results: We successfully ...
The most widely used in vivo model is the pancreatic cancer cell source subcutaneous/orthotopic transplantation tumor model; however, it cannot reflect the interactions between stromal elements with pancreatic cancer cells. Therefore, our research group usually adopted an improved transplant model in ...
Flow cytometric analysis of tumor infiltrating immune populations in mice with co-implanted orthotopic and subcutaneous lesions revealed dichotomies in the density of CD8 T cells, CD4 effector T cells, and dendritic cell populations between tissue sites in response to local therapy.Conclusions: Although...