K. pneumoniae ATCC 700603 and Escherichia coli ATCC 25922 were used as quality control strains for susceptibility testing [27]. Phenotypical identification of HMKP isolates String tests were conducted to determine the hypermucoviscosity phenotype. The presence of the hypermucoviscosity phenotype was ...
similipneumoniae strain ATCC 700603 reference strain. Infection of G. mellonella larvae with ST392 clinical isolates showed that the latter were not highly pathogenic in this model. Together, our results indicate that ST392 isolates have the potential to become a strain of clinical relevance, ...
Plasmids and cell lines will be provided upon request. Experimental model and subject details Cells lines All cells were purchased from ATCC (Molsheim Cedex, France). The 4T1 cell line was cultured in RPMI supplemented with 8% heat-inactivated FCS (v/v) and 2 mM L-glutamine at 37°C and...
denitrificans ATCC 15173. Microbiol Res. 2013, 168: 360-366. 10.1016/j.micres.2013.01.002. Article CAS PubMed Google Scholar Wiame E, Duquenne A, Delpierre G, Van Schaftingen E: Identification of enzymes acting on a-glycated amino acids in Bacillus subtilis. FEBS Lett. 2004, 577: 469...
This plate was pre-inoculated with an Escherichia coli ATCC 25922 and then incubated overnight at 37 °C. The positive result for carbapenemase production was ≤ 15 mm, the negative impact was a clear zone of ≥ 19 mm, and the indetermi- nate result was 18 mm or...
K. pneumoniae ATCC700603 and Eschercihia coli ATCC 25922 were used as quality control strains for the antibiotic susceptibility tests. 2.3. Detection of the hypermucoviscous phenotype All carbapenem-resistant K. pneumoniae isolates were grown in nutritive agar (Britania®) enriched with 5% defi...
similipneumoniae strain ATCC 700603 reference strain. Infection of G. mellonella larvae with ST392 clinical isolates showed that the latter were not highly pathogenic in this model. Together, our results indicate that ST392 isolates have the potential to become a strain of clinical relevance, ...
The AsPC-1, Capan-1, Capan-2 and BxPC-3 cell lines were grown in RPMI 1640 (c.c.pro) supplemented with 10% FCS, 1% P/S, and 1% L-glutamine. 4.2. Viruses CVA21 (strain Kuykendall) was purchased from ATCC (Manassas, VA, USA) and amplified on HeLa cells with 0.3 MOI for 48 h...