The PCR conditions of seven housekeeping genes—adk, fumC, gyrB, icd, mdh, purA, recA for E. coli, and gapA, tonB, rpoB, phoE, mdh, infB, pgi for K. pneumoniae—were amplified and sequenced as described by Wirth et al. (2006). The PCR products were sequenced by the Sanger ...