However, they are tumor cells carrying a gain-of-function mutation of Kit. We established an immortal mast cell line without Kit mutation, NCL-2, derived from NC mouse bone marrow. NCL-2 cells could be maintained without additional growth factors and thus could respond to exogenous growth ...
LUTZKY J, BAUER J, BASTIAN B C. Dose-dependent, complete response to imatinib of a metastatic mucosal melanoma with a K642E KIT mutation[J]. Pigment Cell Melanoma Res, 2008, 21(4): 492-493. RAPISUWON S, PAR...
For most patients, sunitinib will then be used and effective unless one mutation, D816H/V, is present which is resistant to both TKIs. Imatinib works better on inactive CD117 and prevents activation, but does not bind to activated CD11784. Further, SCF in the bone microenvironment alters ...
[Foundation.Register("NSCell", true)] public class NSCell : Foundation.NSObject, AppKit.INSAccessibility, AppKit.INSAccessibilityElementProtocol, AppKit.INSUserInterfaceItemIdentification, Foundation.INSCoding, Foundation.INSCopying, IDisposable继承...
DidChange(NSString, NSKeyValueSetMutationKind, NSSet) (继承自 NSObject) DidChangeValue(String) 指示对指定的键进行了更改。 (继承自 NSObject) Dispose() 释放NSObject 对象使用的资源。 (继承自 NSObject) Dispose(Boolean) DoesNotRecognizeSelector(Selector) 指示此对象无法识别指定的选择器。
Cell culture and chemicals Three human AML cell lines, Kasumi-1 (ATCC, Manassas, VA) with t(8;21) and heterozygous KIT N822K mutation, SKNO-1 (JCRB, Osaka, Japan) with t(8;21) and homozygous KIT N822K mutation, and OCI-AML3 (DSMZ, Brauschweig, Germany) with NPM1 mutation and DN...
A specimen is positive for a given mutation if signal ≥ validated cut off Recommended research use cutoff: 400-500 MFI Analytical sensitivity* of about 1% demonstrated with genomic DNA and model FFPE cell line dilutions Includes internal endogenous control (EC) to assess sample DNA quality/purity...
A specimen is positive for a given mutation if signal ≥ validated cut off Recommended research use cutoff: 400-500 MFI Analytical sensitivity* of about 1% demonstrated with genomic DNA and model FFPE cell line dilutions Includes internal endogenous control (EC) to assess sample DNA quality/purity...
In this study, we examined the impact of a mutation in theextracellular domain of KIT on mast cell tumorigenesis. A KIT mutant with anAsn508Ile variation (N508I) in the extracellular domain derived from a canine mastcell tumor was introduced into IC-2 cells. The IC-2N508I cells...
Notably, a single point mutation in either of these domains results in a nickase enzyme, whereas mutations in both domains (D10A and H840A for SpCas9) results in complete loss of DNA cleavage activity42. Researchers have repurposed these Cas9 variants for a wide range of genome-targeting ...