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Kinase Buffer II(激酶缓冲液II),5X 运输:常温 保存:4-25℃ 有效期:2年 货期:1-3天 产品介绍: 概念: 在溶解过程中发生了两种变化,一种是溶质的分子(或离子)在水分子的作用下向水中扩散,这一过程吸收热量;另一种是扩散的溶质的分子(或离子)和水分子作用,生成水和分子(或水和离子),这一过程放出热量。
Cell Lysis Buffer (10X) #9803 15 ml ATP (10 mM) #9804 50 µl Description Nonradioactive p44/42 MAP Kinase Assay Kit provides all the reagents necessary for measuring p44/42 MAP kinase activity in the cell. Immobilized Phospho-p44/42 MAPK (Thr202/Tyr204) mAb is used to immunoprecipit...
Kinase Buffer (10X) #9802 15 ml Cell Lysis Buffer (10X) #9803 15 ml ATP (10 mM) #9804 50 µl Description The SAPK/JNK Kinase Assay Kit (Nonradioactive) provides all the reagents necessary to measure SAPK/JNK activity in the cell. A phospho-SAPK/JNK Rabbit mAb linked to agarose be...
Cell-free kinase assay was performed in kinase buffer (CST, 9802 S) containing 25 mM Tris-HCl (pH 7.5), 5 mM β-glycerophosphate, 2 mM dithiothreitol, 0.1 mM Na3VO4, and 10 mM MgCl2. The CLK4 protein was incubated with C-NEXN (amino acids 295–671) or C-NEXN-S...
配制 50mM含50% PEG300的磷酸buffer 剂量 50mg/kg,75mg/kg 给药方式 腹腔注射 参考文献: 1. Harrington EA, et al. Nat Med. 2004, 10(3), 262-267. 2. Arlot-Bonnemains Y, et al. Endocr Relat Cancer. 2008, 15(2), 559-568. 3. Young MA, et al. Cancer Res. 2006, 66(2)...
高通量药物筛选利器——HTRF,在激酶研究(kinase)中的应用
CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit. An alternate PTMScan® IAP Buffer Plus Detergent #9992 which may reduce nonspecific interactions is available separately. A detailed protocol and Limited Use License allowing the use of the patented PTMScan® method ...
Parallel whole-cell lysates were generated using CST lysis buffer (20 mM Tris, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM pyrophosphate, 50 mM NaF, 5 mM β-glycero-phosphate, 50 nM calyculin A, 1 mM Na3VO4, and protease inhibitors)...
方法 Aurora B激酶实验中,HeLa细胞溶解在含50mM NaCl的buffer中。使用台式离心机对全部细胞抽提物进行离心,13000rpm 4℃下离心20分钟。200mg全部细胞抽提物在含250mM NaCl的15ml溶解buffer中再次提取,为了从有丝分裂染色质中获得活性Aurora B激酶。第二次提取的低速悬浮液用于免疫沉淀反应。鼠单抗AIM-1或HA与GammaBin...