MCE 国际站:Kinase Assay Buffer 品牌:MedChemExpress (MCE) 存储条件:密闭、避光保存-20°C,2 年 简介:MCE Kinase Assay Buffer 是一种用于检测激酶蛋白的缓冲储备液。 概述:MCE 激酶检测缓冲液 (5×, in ddH2O) 是一种用于检测激酶蛋白的缓冲储备液。本产品包含 20 mM MOPS、25 mM β-glycerol phosphate...
公司名称:Abcam推荐 联系电话:+1 (877) 774-8286 电子邮件:us.orders@abcam.com 国籍:美国 产品介绍:中文名称:Kinase Assay缓冲液I 英文名称:Kinase Assay Buffer I CB指数:50 网址:www.abcam.com 相关信息:全球销售网络产品目录(6000)用户评价 1
For hRIPK1, the kinase assay buffer (2×) contains 50 mM HEPES pH 7.5, 30 mM MgCl2, 50 mM NaCl, 0.5 mg/mL BSA, 0.02% CHAPS and 1 mM DTT. For the Aurora kinases, the kinase assay buffer was used from the promega assay (V9081, V9181). Next, the in vitro ADP-...
The assay kit comes in a convenient 96-well format, with enough purified recombinant CDK16 (amino acids 107-496)/Cyclin Y complex, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions. ● Synonyms Cell division protein kinase 16, PCTAIRE-motif protein kinase 1, Serine/...
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Protein concentration was determined by Bradford assay. Kinase assays Kinase assays were performed as described previously15. In brief, purified CDK complexes were incubated in kinase assay buffer (50 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 0.1% v/v NP-40, 1 mM DTT, 20 μ...
G9291 CytoTox-Glo™ Cytotoxicity Assay 5 X 10ml 4553 G9292 CytoTox-Glo™ Cytotoxicity Assay 2 X 50ml 6974 G9381 Mammalian Lysis Buffer 40ml 541 G9410 HaloCHIP™ System 20 reactions 5555 G9441 Digitonin 40µl 1084 G9451 Protease-Glo™ Assay 1 each 11468 ...
摘要: ItemULight-MBP peptideEu-anti-phospho-MBPLANCE Detection Buffer 10XWhite OptiPlates-384MAP kinase 1/ERK1, activeStaurosporineATPTopSeal-ATMEnVision Multilabel ReaderMirror: LANCE/DELFIATM DualExcitation Filter: UV2(TRF) 320 nm Emission Filter: Eu 615 nmEmission Filter: LANCE 665 nm...
1X Kinase Buffer A + any kinase-specific additives Detection reaction (20 μL final)10 mM EDTA 2 nM phosphospecific antibody TOP Detailed Assay Procedure (1.1)1X Kinase Buffer A:Prepare a 1X solution of Kinase Buffer A from the 5X ...
AKT kinase activity was determined using an AKT kinase assay kit (Cell Signaling Technology). Briefly, AKT was immunoprecipitated according to the manufacturer's instructions, and kinase activity was assessed by measuring phosphorylation of a GSK3 substrate via immunoblot using αpGSK3 Ab. ...