为了揭示 HCC 的细胞靶点,研究人员合成了一种生物素标记的 HCC 探针(Bio-HCC,图 2 A),并通过pull down实验筛选其结合蛋白,发现Keap1被Bio-HCC显著结合(图2B),pull down+WB(图2C),DARTS+WB(图2D),CETSA+WB(图2E),MST(图2F)及分子对接(图2G)均证实HCC与Keap1是直接结合。 图2 Keap1是HCC的直接结合...
60027-1-Ig targets KEAP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Reactivity human, mouse, rat Cited Reactivity human, mouse, rat, pig, chicken Host / Isotype Mouse / IgA Class Monoclonal Type Antibody Immunogen KEAP1...
30041-1-AP targets KEAP1 in WB, ELISA applications and shows reactivity with Human samples. Tested Applications WB, ELISA Application Description Tested Reactivity Human Immunogen KEAP1 fusion protein Ag32512 种属同源性预测 Host / Isotype Rabbit / IgG Class Polyclonal Type Antibody Full Name kelc...
30041-1-PBS targets KEAP1 in WB, Indirect ELISA applications and shows reactivity with human samples. Tested Reactivity human Host / Isotype Rabbit / IgG Class Polyclonal Type Antibody Immunogen KEAP1 fusion protein Ag32512 Full Name kelch-like ECH-associated protein 1 Calculated Molecular Weight...
应用范围:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 产地:上海 品牌:爱必信(absin) 货号:abs115782 用途:见爱必信官网 亚型:见爱必信官网 是否单克隆:见爱必信官网 免疫原:见爱必信官网 保质期:见爱必信官网 标记物:见爱必信官网 ...
WB See 94 publications below IHC See 8 publications below IF See 18 publications below IP See 2 publications below CoIP See 3 publications below 产品信息 60027-1-Ig targets KEAP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested...
作者进一步分析KEAP1-259aa的生物学功能，通过过表达circKEAP及IRES、ATG突变载体/CCK8/流式检测/细胞划痕/Transwell侵袭，发现KEAP1-259aa抑制OS细胞的增殖和克隆形成及调节细胞迁移能力，并通过流式/WB检测相关干细胞标志物的下调及肿瘤球形成试验/裸鼠皮下注射实验发现该蛋白可抑制肿瘤的形成。图4. KEAP1-259aa可...
产品名称 胞质接头蛋白Keap1 产品规格 50ul、100ul、200ul 有效成分含量 98% 是否进口 否 用途范围 仅用于科研实验、不得用于临床 包装 盒 英文名称 Rabbit Anti-KEAP1 antibody 分类 一抗 实验类型 WB,IHC-P,IHC-F,ICC,IF,ELISA 应用范围 Human,Mouse,Rat (predicted: Dog,Pig,Cow,Rabbit) 发货...
WB IP Western blot analysis of extracts from various cell lines using KEAP1 (H436) Antibody. 1/1 Learn more about how we get our images This product is discontinued Inquiry Info. # 4617 Please see our recommended alternatives. ALTERNATE PRODUCT INQUIRY Your Local Representative Your Local Pu...
已有文献报道p62与E3连接酶Cul3的keap1有相互作用,图2A的WB结果显示Myc-Cul3表达细胞中泛素化的p62增加。为了进一步验证p62可以被Cul泛素化,作者构建了包含了标志的Cul3-WT或缺少其C末端的非活性Cul3(Cul3-DN),并共沉淀了Flag-Cul3。 纯化缺少p62前84个氨基酸的His-p62与泛素、免疫沉淀FLAG-CUL3和重组E1/E2...