在ABI 7500仪器上进行KASP基因分型说明书 Manual Running KASP genotyping on the ABI 7500 instrument For Research Use Only. Not for use in diagnostic procedures.
【Objective】This study was aimed to develop a set of kompetitive allele specific PCR (KASP) markers that can be used to distinguish the main cultivated grape cultivars in China, so as to provide the technical support for domestic grape cultivar protection, cultivar registration and market right ...
KASP-brochure
Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): overview of the technology and its application in crop improvement Single nucleotide polymorphism (SNP) data can be obtained using one of the numerous uniplex or multiplex SNP genotyping platforms that combine a ...
quarantine measures for this species must be taken correctly and quickly. In this study, Kompetitive Allele-Specific PCR (KASP) genotyping assays were developed to identifyM. chitwoodi. The developed KASP primers were able to distinguishM. chitwoodifrom other nematode species, as well as detect ...
Six additional input files were later created for correlation analyses: (a) dataset 3 con- sisted of a subset of 100 out of 191 SNPs routinely used by CIMMYT for QC genotyping; and (b) data set 4 to dataset 8 were created from dataset 2 using a MAF of 0.10, 0.20, 0.30, 0.40 and...
Seven of these KASP markers, located in the coding region of a probable leucine-rich repeats receptor-like serine/threonine-protein kinase gene (Capana05g000704), were validated in the 237 accessions, which showed an average accuracy of 82.7%. The genotyping of the seven KASP markers strongly ...
genotyping. The genotyping was performed using the 35 k Axiom® Wheat Breeders Array, following the procedure outlined by Affymetrix (Axiom® 2.0 Assay for 294 samples, P/N 703, 154 Rev. 2). Further to ensure reliable data, SNP markers with > 10% missing data, < 10% MAF, ...
The SNP markers used to construct the genetic maps will be a useful resource in research on radish and should lead to low-cost, accurate, and high-throughput genotyping platforms.doi:10.1007/s13580-020-00253-7Hee-Ju YuYoung-Min Jeong
Phylogenetic analysis of the 15 Korean japonica varieties was performed with genotyping data from 265 KASP markers by the Neighbor-joining method (1000 bootstraps), using POPTREE2 software [34]. 5. Conclusions A set of 1225 polymorphic KASP markers based on SNPs was developed for temperate ...