• qPCR quantifies only amplifiable library molecules;• is accurate across an extremely large dynamic range; and • is amenable to high sample throughput and automated liquid handling.1.71.5 1.3 1.1 0.9 0.7 0.5 0.3 0.1 -0.1 246810121416182022242628303234 Cycle N o r m a l ...
KAPA SYBR FAST qPCR预混液(2X)和ROX/莹光染剂(取决于套件配置)内包含的SYBR Green I染料对光敏感。长时间暴露在直射光下会导致荧光信号强度下降。 KAPA SYBR FAST qPCR Master Mix (2X) 经过30个冻融循环后仍稳定。当不使用时,确保所有试剂在-20℃的温度下避光储存。只要不被微生物和/或核酸酶污染,试剂可...
KAPA SYBR ® FAST qPCR Kits are ideally suited for:• Gene expression analysis • Low copy gene detection • Absolute quantification of NGS libraries.Product Specifications Shipping and Storage Upon arrival, store kit components protected from light at -20 °C in a constant-temperature ...
Complete kits include KAPA SYBR FAST qPCR Master Mix (2X), Primer Premix (10X) and a set of 6 DNA Standards. Reagents for qPCR (KAPA SYBR FAST qPCR Master Mix and Primer Premix) and DNA Standards (1–6) are also sold separately. Where noted, Master Mixes contain instrument-specific refe...
1.2确保KAPA Library Quantifcation Kit的所有组分完全解冻并彻底混合。 1.3如果第一次使用试剂盒,将Primer Premix(10X)(1 mL)加入KAPASYBR®FASTqPCR Master Mix(2X)(5 mL)瓶中。使用涡旋混合器彻底混合。如果您使用的是通用qPCR Master Mix试剂盒,并且仅使用ROX High或ROX Low,则在首次打开试剂盒时,可以使用...
KAPA SYBR ® FAST qPCR Kits are ideally suited or: • Gene expression analysis • Low copy gene detection • Absolute quanti cation o NGS libraries. Product Specifcations Shipping and Storage Upon arrival, store kit components protected rom ...
产品特点Library Quant Kit,Illumina GA revised primers -SYBR Fast Universal二代测序*终文库定量产品 KAPA代理,KAPAKK4824,KAPA全国总代,KAPA Library Quant Kit,Illumina GA revised primers -SYBR Fast Universal二代测序*终文库定量产品 上海易汇生物科技有限公司,是一家集研发、销售为一体的技术生物企业,公司专注...
实时荧光定量PCR(也称为定量PCR或qPCR)是使用聚合酶链式反应(PCR)进行的核酸同步扩增和检测/定量的方法。qPCR是一种可支持您研究应用的多用途的强大工具。查找经过优化的实时荧光定量PCR预混液、试剂和试剂盒,推动您的实验进展。 KAPA SYBR FAST qPCR试剂盒包含第一个通过我们的定向进化技术改造的DNA聚合酶,以提高对...
KAPA SYBR FAST qPCR Master Mix (2X) 中包含的 SYBR Green I染料和ROX/荧光素染料(取决于试剂盒配置)具有光敏性。 长时间暴露在直射光下会导致荧光信号强度下降。 KAPA SYBR FAST qPCR Master Mix (2X) 经过30个冻融循环后仍稳定。当不使用时,确保所有试剂在-20°C的温度下避光储存。如果不被微生物和/或...
The KAPA SYBR® FAST qPCR Kit is supplied as a 2x Master Mix with integrated antibody-mediated hot start, SYBR® Green I fluorescent dye, MgCl2, dNTPs, and stabilizers. ROX reference dye is included in the ABI Prism® Master Mix (2X), and supplied in separate tubes in the Universal...