KAPA SYBR ®Fast qPCR Master Mix (2X) 即使在-20°C 下储存,也可能无法冷冻。 KAPA SYBR FAST qPCR Master Mix (2X) 中包含的 SYBR Green I染料和ROX/荧光素染料(取决于试剂盒配置)具有光敏性。 长时间暴露在直射光下会导致荧光信号强度下降。 KAPA SYBR FAST qPCR Master Mix (2X) 经过30个冻融循环...
KAPA SYBR FAST qPCR预混液(2X)和ROX/莹光染剂(取决于套件配置)内包含的SYBR Green I染料对光敏感。长时间暴露在直射光下会导致荧光信号强度下降。 KAPA SYBR FAST qPCR Master Mix (2X) 经过30个冻融循环后仍稳定。当不使用时,确保所有试剂在-20℃的温度下避光储存。只要不被微生物和/或核酸酶污染,试剂可...
Complete kits include KAPA SYBR FAST qPCR Master Mix (2X), Primer Premix (10X) and a set of 6 DNA Standards. Reagents for qPCR (KAPA SYBR FAST qPCR Master Mix and Primer Premix) and DNA Standards (1–6) are also sold separately. Where noted, Master Mixes contain instrument-specific refe...
KAPA SYBR® FAST qPCR Kit Master Mix (2X) ABI Prism™ KR0390 – v8.13 Product Description KAPA SYBR® FAST qPCR Master Mix is designed for high performance real-time PCR. The kit contains a novel DNA polymerase engineered via a process of molecular evolution. The result is a unique ...
试剂盒包含KAPA SYBR® FAST qPCR Master Mix (2x )、KAPA RT Mix ( 50x )和dUTP。其中KAPA RT Mix ( 50x )包括野生型M-MuLV转录酶、RNase 抑制剂。操作者只需加入引物和RNA即可,一步完成反转录和qPCR两个过程,方便简单快速。适用各种荧光定量PCR 仪,如罗氏LightCyclerR480、ABI PrismR、Bio-Rad iCycler...
仪器配套试剂与SYBR FAST- 致,反应程序在SYBR FAST基础上加入cDNA合成步骤。 1、产品描述 KAPA one -Step qRT-PCR是一款灵敏、方便的定量试剂盒。试剂盒包含KAPA SYBR® FAST qPCR Master Mix (2x )、KAPA RT Mix ( 50x )和dUTP。其中KAPA RT Mix ( 50x )包括野生型M-MuLV转录酶、RNase 抑制剂。操作者...
● KAPA SYBR® FASTqPCR预混液(2x),含有各种被动参比染料(表1)。 表1. 推荐用于KAPA SYBR FAST通用qPCR预混液的ROX浓度 文库定量的方法为:通过使用KAPA SYBR FASTqPCR预混液和靶向Illumina P5和P7流动槽寡核苷酸序列的引物,使用qPCR扩增一组六份预稀释的DNA标准品和稀释的文库样本,进行文库定量。将每份DNA...
Complete (Master Mix) kits include KAPA SYBR FAST qPCR Master Mix (2X), Primer Premix (41 bp, 129 bp and 305 bp, 10X) and a set of 5 DNA Standards. Primer Premixes and DNA Standards are also sold separately. 规格明细 订货信息 ...
KAPA SYBR ® FAST qPCR Master Mix (2X) is free of contaminating DNase and RNase. It is functionally tested by generating a standard curve with human genomic DNA as template, with a dynamic range of 5 orders of magnitude, a reaction efficiency of 90 – 110% and a correlation coefficient ...
通用型SYBR快速定量试剂盒 货号:A-KK4600 KAPA SYBR® FAST Universal 2X qPCR Master Mix 背景资料:The KAPA SYBR® DNA Polymerase was engineered to perform optimally in stringent real-time qPCR reaction conditions, exhibiting dramatic improvements to signal-to-noise ratio (fluorescence), cycle ...