1、Hu, H. and S.C. Sun,Ubiquitin signaling in immune responses. Cell Res, 2016.26(4): p. 457-83. 2、Zeng, W., et al.,Reconstitution of the RIG-I pathway reveals a signaling role of unanchored polyubiquitin ...
Modification of proteins by addition of K63-linked polyubiquitin chains is implicated in a variety of cellular events, including DNA repair, signal transduction and receptor endocytosis. A monoclonal antibody (mAb) has been generated that specifically recognizes K63-linked polyubiquitin chains, but not...
Ub signaling is diversified in part by the capacity for lysine residues on Ub to become sites of ubiquitination, resulting in polyubiquitin (polyUb) chains. PolyUb chains linked through different lysine residues act as distinct signals, targeting the ubiquitinated substrate to different pathways and ...
2023年8月15日,山东大学基础医学院高成江教授团队在Cellular & Molecular Immunology杂志发表题为MAVS-loaded unanchored Lys63-linked polyubiquitin chains activate the RIG-I-MAVS signaling cascade的研究成果。报道了MAVS承载的非锚定K63...
The other type of ubiquitin polymer involves monomers that are linkedlinearly via an initiator Met (linear polyubiquitin). Polyubiquitin’s functions depend on which Lysresidue is involved in cross-linking the protein. K63-linked polyubiquitin chains exhibitnonproteolytic function in vivo which includes...
NEMO itself is modified by the K63-linked polyubiquitin chains. It remains open whether NEMO can bind to itself through these conjugated K63-linked polyubiquitin chains to form oligomers of IKK complex. Previous studies indicated that NEMO could form a dimer, trimer or tetramer in vivo and in ...
This domain binds efficiently to K63-linked poly-ubiquitin chains within a narrow range of chain lengths but not to K48-linked poly-ubiquitin chains. Other syntaxin family members also bind to K63-linked polyubiquitin chains but with different chain length specificities. Molecular modeling suggests ...
Biotin K63-TUBE consists of multiple ubiquitin interacting motifs separated by rigid linkers, presumably increasing its affinity to extended K63 polyubiquitin chains. Biotin K63-TUBE is useful for detection and characterization of K63-linked polyubiquitylated proteins by Far Western blotting, affinity pu...
1 Although K63-linked chains have been associated with the regulation of vesicle trafficking, definitive proof for their role in endocytosis was missing. We show that the ubc35 ubc36 mutant has pleiotropic phenotypes related to hormone and immune signaling. Specifically, we reveal that ubc35-1 ...
Structures of the K63-linked triubiquitin chains[J]. Acta Crystallographica Section D: Biological Crystallography, 2008, 64(Pt 3): 1082-91. 2. Nathan J A, Kim H T, Ting L, et al. Why do cellular proteins linked to K63-polyubiquitin chains not associate with proteasomes?[J]. The ...