The invention discloses a cell strain with specific expression of HLA-G1 antigen. The preservation number is CCTCC: C201351. The preservation date is on May 8, 2013. The preservation place is China Center for Type Culture Collection. The molecular weight of the HLA-G1 antigen produced by ...
摘要本研究目的是建立稳定表达HLA-A * 1101分子的K562细胞株,为研究慢性髓系白血病(CML)HLA-I限 制性抗原特异T细胞的细胞毒作用提供靶细胞。利用RT-PCR从CML患者外周血单个细胞中扩增HLA-A * 1101 基因全长序列;利用重组PCR将2A肽连接子(D-V-E-X-N-P-G-P)基因连接到HLA-A ...
HLAI类分子无关;MICA/B在多药耐药肿瘤细胞的低表达导致耐药肿瘤细胞对NK细胞杀伤敏感性下降。 关键词 自然杀伤细胞;MHCI类链相关分子A/B;K562细胞 中图分类号 R733.7;R730.54 文献标识码 A Expression0fHLAClassIMoleculesandMHCClassIChain-re- latedMoleculesA/Binl<562andl<562/A02CellLinesandTheir ...
Expression of HLA C lass I M olecules and MHC C lass I Chain re lated M olecules A /B in K 562and K 562/AO 2Cell L ines and Their E ffects on Cytotoxicity ofNK C ells M EI Jia Z huan,N I U X in Q ing,GUO Kun Y uan,ZHOU Jian,WE I H ong M ei D epart...
目的:与经典HLA I类分子相比,HLA-G分子的组织分布有限.甲基化导致的基因沉默现象是表观遗传学领域研究的热点之一,本文研究K562细胞系HLA-G基因启动子区甲基化状态及其与基因表达的关系.方法:应用亚硫酸氢钠脱氨基-DNA测序(bisulphite sequencing)的方法检测经5-氮胞苷(5-aza-2'-deoxy- cytidine)处理前后的K562细胞...
This cell line lacks surface expression of human leukocyte antigen (HLA) class I and II molecules. Unlike magnetic beads, K562 can be readily manipulated to express additional co-stimulatory molecules by viral transduction [60,61]. We initially engineered K562 cells to express the low-affinity Fc...
5、r 96 h treatment with A23187 (375ug/ml) and PMA (10 g/mL), the cells exhibited the characteristic DC morphology, CD83, CD86, CD80, CD40 and HLADR, CD1a expression was upregulated and the cells were found to be able to strongly stimulate the proliferation of allogeneic T cells when...
PMA联合钙离子载体诱导K562细胞向树突状细胞分化目的探讨卟啉醇肉豆蔻酸乙酸酯,PMA,联合钙离子载体,CI,诱导K562细胞向树突状细胞,DC,分化的作用,方法对数生长期的K562细胞在含PMA和CI的培养液中培养96h后,倒置相差显微
.TheproliferationreactionoflymphocytecellswasdetectedbyMTTcolorimetry.ResultsAfter96htreatmentwithA23187(375ug/ml)andPMA(10μg/mL),thecellsexhibitedthecharacteristicDCmorphology,CD83,CD86,CD80,CD40andHLA DR,CD1aexpressionwasupregulatedandthecellswerefoundtobeabletostronglystimulatetheproliferationofallogeneicTcells...
胞对K562/AO2细胞杀伤活性降低与HLAI类分子无关;MICA/B在多药 耐药肿瘤细胞的低表达导致耐药肿瘤细胞对NK细胞杀伤敏感性下降 关键字:自然杀伤细胞;MHCI类链相关分子A/B;K562细胞 ExpressionofHLAClassIMoleculesandMHCClassI ChainrelatedMoleculesA/BinK562andK562/AO2CellLines ...