DSMZ Cell Culture Data: Morphology:round large, single cells in suspension; Medium:90% RPMI 1640 + 10%FBS 购买gibco血清,送培养基,送好礼,详情关注微信! Subculture: maintain at 0.1-0.5 x 106 cells/ml; split 1:3 to 1:5 every 3 days; seed out at ca. 0.3-0.5 x 106 cells/ml; viabil...
英文名称:K562 Cells | K562 Human Chronic Myeloid Leukemia Cell Culture Medium 英文同义词:K562 Cells | K562 Human Chronic Myeloid Leukemia Cell Culture Medium CAS号: 分子式: 分子量:0 EINECS号: 信息错误报告 您的Email: 按国家浏览K562 Cells | K562 Human Chronic Myeloid Leukemia Cell Culture Medium...
K562 erythroleukemic cells cultured at low population density in the absence of serum die within 12鈥 24 hours, unless 0.1 mM glyoxylic acid is added to the culture medium. Earlier events, preceding cell death and occurring within 2 hours culture, are: a) a marked drop of both the NAD +...
CAS号: 英文名: K562 Cells | K562 Human Chronic Myeloid Leukemia Cell Culture Medium 英文别名: K562 Cells | K562 Human Chronic Myeloid Leukemia Cell Culture Medium 中文名: K562 细胞| K562 人慢性髓原白血病细胞 专用培养基 中文别名: K562 细胞| K562 人慢性髓原白血病细胞 专用培养基 CBNumber:...
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. lncubate cultures at 37℃.操作流程1.吸走多余培养基,加入3-5mL PBS后轻轻晃动培养瓶润洗细胞; 2.吸干净PBS后加入1mL 0.25%胰酶...
优利科(上海)生命科学有限公司供应K562 人慢性髓原白血病细胞供应产品,K562人慢性髓原白血病细胞ProductFormat:a15mlcentrifugetubeCultureProperties:悬浮CompleteGrowthMedium:IMDM+10%FBS+1%双抗Atmosphere:air,95%;carbondioxide(CO2),5%Application:Cellsandcancerr
K562 in 20ul tubes Cell Number:1-2*10^6 cells Plasmid:2ug E buffer:Celetrix buffer Suggested parameter:Cell Line Mode,480V-520V Culture medium:1640+10%FBS (antibiotics free) K562 in 100ul tubes Cell Number:5-10*10^6 cells Plasmid:10ug ...
HL-60 cells are a promyelocytic cell line that can be induced to diffentiate along the granulocytic or monocytic/macrophage lineage by a variety of compounds, such as DMSO, TPA, and retinoids [13]. Unlike other differentiating agents, HNE is highly unstable in culture medium (it disappears ...
ObjectiveTo obtain the exosomes from the culture media of K562 cells.To construct and express CD63-GFPfusion gene in K562 cells.MethodsExosomes from K562 culture medium were obtained by ultracentrifugation,and themorphology of exosomes were observed under a transmission electron microscope.CD63 was ...
ABSTRACT:ObjectiveToexploretheeffectofPMA(phorbolmyristateacetate)andcalciumionophore(CI)ininducingthedifferentiationofleukemiacelllineK562intoactivateddendriticcells(DC).MethodsK562cellswereculturedinacommonmediumcontainingCI(A23187)andPMAfor96h.Thecellmorphologywasobservedunderthelightphasecontrastmicroscopeandtheelectroni...