Cell-free culture media conditioned by all but two of the seven types of monolayer studied inhibited haemoglobin synthesis by K562 cells; those conditioned by blood-monocyte-derived macrophages and two of 11 monolayers of bone-marrow-derived macrophages stimulated haemoglobin synthesis. Thus, the ...
The alterations in glucose concentration of the culture media were monitored while the apoptosis of K562 cells was detected by flow cytometry. The effects of high concentration insulin on the proliferation of K562 cells were inhibited by varying concentrations of insulin-like growth factor-1 (IGF-1...
The ability of 26S proteasomes from the human proerythroleukaemic cell line K562 to degrade high-molecular-weight cytoplasmic RNAs, particularly specific messenger RNA, has been detected. The addition of hemin to K562 cells in the culture media leads to redistribution of proteasomes and their migra...
media, containing 10% FBS on ice for 5 min. CFSE-labeled exosomes were diluted in 60 ml PBS, collected by ultracentrifugation (100,000 × g for 70 min at 4°C) and resuspended in 1.5 ml cell culture media with K562 cells (500,000 cells/ml). CFSE-positive cells were observed under ...
Light-dependent generation of reactive oxygen species in cell culture media. Free Radic Biol Med 30, 1418–1425, https://doi.org/10.1016/S0891-5849(01)00545-7 (2001). Article CAS PubMed Google Scholar Salati, S. et al. Role of CD34 antigen in myeloid differentiation of human ...
S2). To be noted, because of its poor solubility in cell culture media, a large amount of this compound would be dissolved out22, the accurate anti-leukemia results of indirubin could not be achieved by cell viability assays in vitro. As for the other two components, isorhamnetin (Fig. 3...
Cell Culture—K562, a human erythroleukemia cell line, was grown in RPMI supplemented with 10% fetal calf serum, streptomycin (50 μg/ml), and penicillin (50 units/ml). Exosome Isolation—Exosomes were collected from 10 ml of K562 media (15–20 × 106 cells) cultured over 7–15 h. Th...
Therefore, coculture systems of irradiated feeder cells and NK cells in media containing IL-2 and IL-15 have been developed to generate large numbers of NK cells, although NK cell expansion protocol using anti-CD3 antibody (OKT-3) without feeder cells has also been developed. Commonly used ...
Cell nuclear lysates from K562 cells were probed by immunoblotting with anti-WT1 antibody (A). Coomassie blue stain was used as a quantity control (B). The bands were quantified by scan densitometry (C). The control culture for this experiment was cells treated with 0.01% Me2SO (vehicle ...
Media were added to integrated cells and it was evenly dispersed via pipette. Five milliliters of medium containing cancer cells were transferred to the cell culture flask, and it was cultured for further expe... DI Kim - 《J.japan Inst.light Metals》 被引量: 5发表: 2006年 The study ...