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Cell Culture—K562, a human erythroleukemia cell line, was grown in RPMI supplemented with 10% fetal calf serum, streptomycin (50 μg/ml), and penicillin (50 units/ml). Exosome Isolation—Exosomes were collected from 10 ml of K562 media (15–20 × 106 cells) cultured over 7–15 h. Th...
S2). To be noted, because of its poor solubility in cell culture media, a large amount of this compound would be dissolved out22, the accurate anti-leukemia results of indirubin could not be achieved by cell viability assays in vitro. As for the other two components, isorhamnetin (Fig. 3...
Therefore, coculture systems of irradiated feeder cells and NK cells in media containing IL-2 and IL-15 have been developed to generate large numbers of NK cells, although NK cell expansion protocol using anti-CD3 antibody (OKT-3) without feeder cells has also been developed. Commonly used ...
After 7d culture, NK cells increased to 71.7 ± 3.9%, compared to media alone (9.7 ± 2.4%) and WTK562 (42.6 ± 5.9%,p < 0.01). This represented a 35-fold or 3374 ± 385% increase of input NK cell number. CB Tcells were decreased compared to media alone and WTK562 (15.1 ± ...
media, containing 10% FBS on ice for 5 min. CFSE-labeled exosomes were diluted in 60 ml PBS, collected by ultracentrifugation (100,000 × g for 70 min at 4°C) and resuspended in 1.5 ml cell culture media with K562 cells (500,000 cells/ml). CFSE-positive cells were observed under ...
Tissue culture Human chronic myelogenous leukemia cells (K562, ATCC) were maintained at 37 oC in 5% CO2between 0.1–1 million cells/ml in DMEM (Dulbecco’s Modified Eagle Media) containing 10% bovine calf serum and 1% Penicillin/Streptomycin. ...
Natural Killer cells have shown promise to treat different malignancies. Several methods have been described to obtain fully activated NK cells for clinical use. Here, we use different cell culture media and different artificial antigen presenting cells to optimize a GMP compliant manufacturing method ...
The human chronic myeloid leukemia cell line K562 acquires several megakaryoblastoid features when cultured in the presence of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA). We observed strongly increased secretion of several proteins into the culture media of K562 cells within a ...
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