ubiquitinationUbiquitination and deubiquitination have a critical role in protein homoeostasis in the cell. Here, we have characterized a novel USP44 (ubiquitin-specific protease 44), which has a ZnF-UBP (zinc-finger ubiquitin-specific protease) domain and conserved cysteine, histidine and asparagine/...
今天给大家讲解一个可视化检测K48泛素化的技术:泛素化诱导的荧光互补(ubiquitination-induced fluorescence complementation,UiFC)。 泛素是一种76个氨基酸的蛋白质,在所有真核生物中高度保守。它可以作为单个部分或以泛素链的形式与蛋白质偶联。泛素分子上有7个赖氨酸位点:K6、K11、K27、K29、K33、K48、K63可以与目...
The ROS-dependent TRAF6-mediated non-proteolytic, K48/63-linked ubiquitination of ATG9A enhances its association with Beclin 1 and the assembly of VPS34-UVRAG complex, thereby stimulating autophagy. Notably, expression of the ATG9A ubiquitination mutants impairs ROS-induced VPS34 activation and ...
Ubiquitination and deubiquitination have a critical role in protein homoeostasis in the cell. Here, we have characterized a novel USP44 (ubiquitin-specific protease 44), which has a ZnF-UBP (zinc-finger ubiquitin-specific protease) domain and conserved cysteine, histidine and asparagine/aspartic acid...
Our findings provide important insights into how K48/K63-linked ubiquitination of ATG9A contributes to the regulation of oxidative stress-induced autophagy. 展开 关键词: ATG9A TRAF6 VPS34 complex autophagy oxidative stress DOI: 10.1016/j.celrep.2022.110354 ...
A proteomics approach to understanding protein ubiquitination Nat. Biotechnol., 21 (2003), pp. 921-926 View in ScopusGoogle Scholar Phu et al., 2011 L. Phu, A. Izrael-Tomasevic, M.L. Matsumoto, D. Bustos, J.N. Dynek, A.V. Fedorova, C.E. Bakalarski, D. Arnott, K. Deshayes, ...
这次分享的文献题目是:CHIP-mediated CIB1 ubiquitination regulated epithelial-mesenchymal transition and tumor metastasis in lung adenocarcinoma,影响因子为15.820分,发表在Cell Death & Differentiation杂志上,发表时间为2020年10月,该文章主要探究了CHIP介导CIB1泛素化过程,从而调控肺腺癌的上皮-间质转化和肿瘤的转移。
the proteins in cluster 2 show a propensity to interact with the shorter K63-linked Ub2present in the branch of (Ub)2–48,63Ub–48Ub. Proteins in these two clusters are strongly linked to biological processes associated with K63-linked ubiquitination, including protein unfolding and refolding,...
方法是we performed the cycloheximide(CHX) assay and pulled down K33-, K63-, or K48-ubiquitinated TBK1. 这样就发现他们的假设是正确的。 最后的最后,也就是我们的结果 USP38 inhibits type I IFN signaling by removing K33-linked and promoting K48-linked poly-ubiquitination chains of TBK1 at Lys...
Esophageal squamous cell carcinoma (ESCC) is a common fatal malignant tumor of the digestive tract; however, its pathogenic mechanism is unknown and lacks specific molecular diagnosis and treatment. Therefore, it is particularly important to identify new