今天给大家讲解一个可视化检测K48泛素化的技术:泛素化诱导的荧光互补(ubiquitination-induced fluorescence complementation,UiFC)。 泛素是一种76个氨基酸的蛋白质,在所有真核生物中高度保守。它可以作为单个部分或以泛素链的形式与蛋白质偶联。泛素分子上有7个赖氨酸位点:K6、K11、K27、K29、K33、K48、K63可以与目...
Thus, LDLR can be targeted to the lysosome by either K48 or K63 linkages. We further demonstrate that although both ubiquitin conjugating enzyme E2 (UBE2)Ds and UBE2N/V1 can catalyze LDLR ubiquitination in a cell-free system, UBE2Ds appear to be the major E2 enzymes employed by IDOL in...
这次分享的文献题目是:CHIP-mediated CIB1 ubiquitination regulated epithelial-mesenchymal transition and tumor metastasis in lung adenocarcinoma,影响因子为15.820分,发表在Cell Death & Differentiation杂志上,发表时间为2020年10月,该文章主要探究了CHIP介导CIB1泛素化过程,从而调控肺腺癌的上皮-间质转化和肿瘤的转移。
To gain insights into the structure of K48–K63-branched chains, we determined the crystal structure of the branched trimer (UbK48R, K63R)2–48,63Ub1–72(Table1). In this K48–K63-branched Ub3structure, the K48-linked Ub adopts a closed conformation with interactions between the two...
The ROS-dependent TRAF6-mediated non-proteolytic, K48/63-linked ubiquitination of ATG9A enhances its association with Beclin 1 and the assembly of VPS34-UVRAG complex, thereby stimulating autophagy. Notably, expression of the ATG9A ubiquitination mutants impairs ROS-induced VPS34 activation and ...
泛素化(Ubiquitination)作为一种重要的蛋白质翻译后修饰方式,通过泛素 - 蛋白酶体系统(Ubiquitin-Proteasome System)对肿瘤进展相关蛋白进行调控,这使得 E3 泛素连接酶成为潜在的抗肿瘤靶点。TRIM8 作为 TRIM 家族的成员以及一种 E3 连接酶,在多种疾病和肿瘤发生过程中发挥作用,但其在肺癌中的真正角色一直存在争议,尚...
Esophageal squamous cell carcinoma (ESCC) is a common fatal malignant tumor of the digestive tract; however, its pathogenic mechanism is unknown and lacks specific molecular diagnosis and treatment. Therefore, it is particularly important to identify new
(K48)-linked ubiquitin chain is one of the most abundant chains and a major proteasome-targeting signal in cells. Despite recent advancements in imaging linkage-specific polyubiquitin chains, no tool is available for imaging K48 chains in live cells. Here we report on a ubiquitination-induced ...
2G). Interestingly, TRIM30 overexpression increased IL-22-induced Sox17 ubiquitination, whereas TRIM30 knockdown decreased IL-22-induced Sox17 ubiquitination (Fig. 2h and i). These data suggest that TRIM30 interacts with and ubiquitinates Sox17, and IL-22-induces Sox17 ubiquitination via TRIM30...
方法是we performed the cycloheximide(CHX) assay and pulled down K33-, K63-, or K48-ubiquitinated TBK1. 这样就发现他们的假设是正确的。 最后的最后,也就是我们的结果 USP38 inhibits type I IFN signaling by removing K33-linked and promoting K48-linked poly-ubiquitination chains of TBK1 at Lys...