TRIM31 directly interacts with p53, and inducing the K63-linked ubiquitination of p53 via its RING domain, Meanwhile, TRIM31 suppresses the MDM2-mediated K48-linked ubiquitination of p53 through competitive inhibiting the interaction of MDM2 and p53, leading to the p53 stabilization and activation....
Esophageal squamous cell carcinoma (ESCC) is a common fatal malignant tumor of the digestive tract; however, its pathogenic mechanism is unknown and lacks specific molecular diagnosis and treatment. Therefore, it is particularly important to identify new
3) The UL24 protein induced the degradation of IRF7 via ubiquitination. After the DEFs were treated with the ubiquitin proteasome inhibitor MG132, the degradation of IRF7 by the UL24 protein was alleviated. Coimmunoprecipitation results revealed that DPV UL24 induced the K48/K63-linked ...
We find that, upon ROS stimulation, TRAF6 mediates K48/K63-linked ubiquitination of ATG9A, enhancing ATG9A-Beclin 1 interaction, PI3KC3/VPS34 activity, and autophagy activation. These results reveal a role for ATG9A ubiquitination in regulating oxidative stress-induced autophagy. Results TRAF6 ...
泛素化(Ubiquitination)作为一种重要的蛋白质翻译后修饰方式,通过泛素 - 蛋白酶体系统(Ubiquitin-Proteasome System)对肿瘤进展相关蛋白进行调控,这使得 E3 泛素连接酶成为潜在的抗肿瘤靶点。TRIM8 作为 TRIM 家族的成员以及一种 E3 连接酶,在多种疾病和肿瘤发生过程中发挥作用,但其在肺癌中的真正角色一直存在争议,尚...
Proteins in these two clusters are strongly linked to biological processes associated with K63-linked ubiquitination, including protein unfolding and refolding, autophagy and protein sorting and endosomal transport (Figs. 2b and 3a). These include annotated K63-binding proteins such as the BRCA1 ...
2F). Furthermore, endogenous Co-IP experiments showed that time-dependent K48-linked ubiquitination of Sox17 was greater in IL-22 treated cells than in control cells (Fig. 2G). Interestingly, TRIM30 overexpression increased IL-22-induced Sox17 ubiquitination, whereas TRIM30 knockdown decreased IL...
Proteins can be modified with eight homogenous ubiquitin chains linked by an isopeptide bond between the C-terminus of one ubiquitin and an amine from one of the seven lysines or the N-terminal methionine of the next ubiquitin. These topologically distin
方法是we performed the cycloheximide(CHX) assay and pulled down K33-, K63-, or K48-ubiquitinated TBK1. 这样就发现他们的假设是正确的。 最后的最后,也就是我们的结果 USP38 inhibits type I IFN signaling by removing K33-linked and promoting K48-linked poly-ubiquitination chains of TBK1 at Lys...
K48- and K63-linked polyubiquitination of deubiquitinating enzyme USP44.deubiquitinating enzymegene expressionin situ hybridizationubiquitinationUbiquitination and deubiquitination have a critical role in protein homoeostasis in the cell. Here, we have characterized a novel USP44 (ubiquitin-specific protease ...