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NameTypeDescriptionRequired K2 URL string Your K2 URL (Example: https://[K2ID].onk2.com). True Username securestring The username for this api True Password securestring The password for this api TrueThrottling Limits展开表 NameCallsRenewal Period API calls per connection 100 60 secondsActions...
[41]or by a two-hybrid screen using the SnrK1 protein, a plant conserved homolog of AMPK/Snf1, as a bait[42]. These kinases are phosphorylating a peptide derived fromArabidopsisSnrK1[41], and were shown to regulate SnrK1 activity by phosphorylating its activation loop[42]. As noted above...
SPOP, an E3 ubiquitin ligase adaptor, can act either as a tumour suppressor or a tumour promoter. In prostate cancer (PCa), it inhibits tumorigenesis by degrading several oncogenic substrates. SPOP is the most altered gene in PCa (~15%), which renders it
Considering the lack of traditional drug targets in the Hippo pathway, we decided to focus on identifying kinases, which were potentially actionable targets of pharmacological inactivation. Of the 14 targets identified only one matched this criteria, NUAK2. ...
Stabilization of MT by the drug Taxol prevents new MT growth, and also inhibits ER tubule extension (Terasaki and Reese, 1994). Motor-mediated ER “sliding” movement occurs on stable acetylated MT (Friedman et al., 2010). Motor-independent ER tubule extension along growing MT plus ends is ...
Findings herein demonstrate no obvious effect of psychotherapeutic drug exposure on NTRK2 (or GRIN1, GRM8, GRIP1, SLC1A1) expression. Finally, tissue-related physical factors were considered as possible sources of variance in these experiments. However, there were no significant differences between ...
In the second in vitro screen we also included 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate, previously suggested to be PCK2 inhibitor based on structure, which emerged as the top hit. The specificity of this compound was tested in PCK1 and PCK2 enzymatic assays and showed IC50of 500nM and...
Importantly, the fact that pelitinib, although less effective in vitro in inhibiting hNek2 kinase activity, was more effective in inhibiting the distant seeding phenomenon in vivo further reinforces the need to include in vivo models in early drug discovery efforts. On the basis of our studies...
RTK-Tel Library screen identifies NTRK2 as a vulnerability for PTEN-deficient cells. (a) Immunoblot analysis of PI3K proteins after PTEN knockdown. Activation of PI3K/mTOR pathways is shown with increased p-Akt 473 in the absence of IL3. (b) PTEN knockdown leads to IL3-independent growth. ...