在进入外周血之前,T细胞发育根据细胞表面CD4和CD8分子的表达分为3个阶段:即CD4CD8双阴性(doublenegative,DN)、CD4CD8双阳性(doublepositive,DP)、CD4或CD8单阳性(CD4orCD8singlepositive,SP)。T细胞从DP向SP的转化依赖于细胞表面txl3T细胞表面受体(T—cellreceptor,TCR)的表达以及和功能性胸腺上皮细胞MHC的结合。随后...
Detection of GABA-A channel subunit proteins in CD4+ and CD8+ T cells from rats, mice and the Jurkat cell line.Suresh, K. MenduAmol, BhandageZhe, JinBryndis, Birnir
在人类肿瘤和老鼠模型中,Tal1高表达与胸腺细胞CD4+CD8+阶段分化阻滞相关,而Jurkat细胞是CD4+CD8+T-ALL细胞,而且TAL1 异常表达,在多种T-ALL分子研究中被应用[2, 5],所以在我们的研究中应用了Jurkat 细胞作为研究对象。Jurkat 细胞属于WT型Tal1高表达的细胞株,为了更确切的说明Tal1对T-ALL细胞增殖的影响,下一...
人T淋巴细胞系Jurkat细胞最初来源于一名14岁T-ALL初次复发的男性患者。Jurkat 细胞免疫表型:CD2+、CD3+、CD4+、CD6+、CD7+、CD8-、CD19-、CD34+、TCRα/β+、 TCRγ/δ-。Jurkat细胞是亚四倍体,细胞遗传学特点:87(7 君,已阅读到文档的结尾了呢~~ ...
The response of the human CD4+ T-cell line Jurkat to infection with vaccinia virus was investigated. Virus titers peaked approximately 3 to 4 days after infection, while cell growth paralleled that of uninfected cells, indicating that growth rates were not appreciably affected by viral infection....
as well as the drug resistance to cytarabine (Ara-C) and daunorubicin (DNR) without changing the original CD2+CD3+CD4+dimCD8-CD34-CD45+dim phenotype, compared to uncoated PCL scaffold and tissue culture plate systems. Molecularly, increased chemoresistance is associated with the upregulation of ...
Accumulating evidence suggests that Vav1 is involved in the regulation of cell apoptosis. For example, during negative selection, Vav1 promotes antigen-induced thymocyte apoptosis, and inhibitors of the actin cytoskeleton or protein kinase C (PKC) reverse the effect[20]. In activated CD4+ T cells...
Metabolic reprogramming is critical for T cell fate and polarization and is regulated by metabolic checkpoints, including Myc, HIF-1α, AMPK and mTORC1. Our objective was to determine the impact of mycophenolic acid (MPA) in comparison with rapamycin (Rapa), an inhibitor of mTORC1, on the ...
CD28 is a homodimeric and transmembrane protein expressed by T cells. Nearly all human CD4+ T cells and 50% of human CD8+ T cells express CD28. The CD28 interaction with CD80 (aka B7-1) or CD86 (aka B7-2) on APCs, in conjunction with TCR engagement, triggers a co-stimulation sign...
We and others have shown that adoptive immunotherapy using A/B TCR expressing cytotoxic T cells will augment the eradication of tumor in a SCID model (11, 12). The capability of CTLs to cause tumor regression is thought to be far greater than a humoral response or a CD4 -mediated Th ...