CD8分子的表达分为3个阶段:即CD4CD8双阴性(doublenegative,DN)、CD4CD8 双阳性(doublepositive,DP)、CD4或CD8单阳性(CD4 orCD8 singlepositive,SP)。 T细胞从DP向SP的转化依赖于细胞表面txl3T细胞表面受体(T—cellreceptor, TCR)的表达以及和功能性胸腺上皮细胞MHC的结合。随后成熟的DP细胞继续发 育,表达高密度的...
Nearly all human CD4+ T cells and 50% of human CD8+ T cells express CD28. The CD28 interaction with CD80 (aka B7-1) or CD86 (aka B7-2) on APCs, in conjunction with TCR engagement, triggers a co-stimulation signal (signal 2). It results in T cell proliferation, cytokine production...
Nearly all human CD4+ T cells and 50% of human CD8+ T cells express CD28. The CD28 interaction with CD80 (aka B7-1) or CD86 (aka B7-2) on APCs, in conjunction with TCR engagement, triggers a co-stimulation signal (signal 2). It results in T cell proliferation, cytokine production...
Detection of GABA-A channel subunit proteins in CD4+ and CD8+ T cells from rats, mice and the Jurkat cell line.Suresh, K. MenduAmol, BhandageZhe, JinBryndis, Birnir
We and others have shown that adoptive immunotherapy using A/B TCR expressing cytotoxic T cells will augment the eradication of tumor in a SCID model (11, 12). The capability of CTLs to cause tumor regression is thought to be far greater than a humoral response or a CD4 -mediated Th ...
在人类肿瘤和老鼠模型中,Tal1高表达与胸腺细胞CD4+CD8+阶段分化阻滞相关,而Jurkat细胞是CD4+CD8+T-ALL细胞,而且TAL1 异常表达,在多种T-ALL分子研究中被应用[2, 5],所以在我们的研究中应用了Jurkat 细胞作为研究对象。Jurkat 细胞属于WT型Tal1高表达的细胞株,为了更确切的说明Tal1对T-ALL细胞增殖的影响,下一...
人 T 淋巴细胞系 Jurkat 细胞最初来源于一名 14 岁 T-ALL 初次复发的男性患者。Jurkat 细胞免疫表型:CD2+、CD3+、CD4+、CD6+、CD7+、CD8-、CD19-、CD34+、TCRα/β+、 TCRγ/δ-。Jurkat 细胞是亚四倍体,细胞遗传学特点:87(78-91) 4nXX, -Y, -Y, -5, -16, -17, -22, (2)(p21)/del(2...
由表2可见经DC激发的T细胞有明显上调CD3、CD4、CD8、CD28分子的作用(因检测例数少,未进一步作统计学分析)。ELISA法定量检测IL-12的含量为61 μg/L。Table 2. Phenotype of T lymphocytes effected by DC(略) 致敏DC诱导CTL细胞毒作用的观察 用LDH释放法检测CTL的细胞毒效应,结果见表3。用配对t检验,对实验组...
The early lesions are hypercellular but become cytopenic when plaque cells undergo necrosis or apoptosis, suggesting that cell death occurs during the disease pathogenesis and could contribute to lesion development (9). Activated T-cells are present in early atherosclerotic areas and may constitute up...