原代T细胞或PBMC及Jurkat T 细胞激活测试(T Cell Activation Assay) T细胞或PBMC活化测定试验确定受试品对T细胞或PBMC在细胞活化过程中的调节作用。 东大生物的方法可以评估测试品在由CD3 / CD28抗体诱导下的T细胞或PBMC 活化中的作用,测试方式包括用细胞因子的ELISA或流式细胞仪(FCM)的测定。 案例一:原代T细胞激活后...
Alteration of CD3 and CD28 activation pathways in CD2-jurkat cell variantsdoi:10.1016/0198-8859(88)90199-1H. Makni and J. Malter and J. Reed and T. Williams and J. Burlein and J. Bender and J. Kant and M. KamounHuman Immunology...
T Cell Activation. Ann. Rev. Immunol. 27:591-619. Figures Validation of CD3 and CD28 expression (flow cytometry) Dose-dependent responses to antibody-mediated CD3 and CD28 cross-linking Back to the top Specifications Growth medium: IMDM, 2 mM L-glutamine, 25 mM HEPES, 10% (v/v) ...
Ligation of the CD2 cell surface glycoprotein expressed on T lymphocytes and NK cells induces protein tyrosine phosphorylation and activation of the Src ki... PD King,S Ali,A Han,... - 《International Immunology》 被引量: 52发表: 1996年 TIM-3 Suppresses Anti-CD3/CD28-Induced TCR Activation...
2. Macian F., 2005.NFAT proteins: key regulators of T-cell development and function. Nat Rev Immunol. 5(6):472-484. Figures Validation of human CD3 and absence of CD28 expression NFAT activation upon stimulation using small molecules
However, upon activation the involvement of CD5 is the opposite in the CD2 and CD3 pathways. CD5 becomes rapidly tyrosine phosphorylated after CD3 stimulation, but is dephosphorylated upon CD2 cross-linking. These opposing effects correlate with the decrease in the activity of the SH2 domain-...
目的探讨CD3AK细胞诱导白血病Jurkat细胞凋亡的效果.方法用细胞形态学观察,DNA琼脂糖电泳,原位末端标记法分别检测Jurkat自然凋亡率和CD3AK细胞诱导的Jurkat细胞凋亡率.结果 Jurkat细胞自然凋亡率为(4.60±2.17)%;CD3AK细胞诱导的Jurkat细胞凋亡率为(27.38±4.91)%,两者之间具有显著性差异(t=15.1P<0.01).结论 CD3AK细胞能...
32922: Yang RY, et al. Expression of galectin-3 modulates T-cell growth and apoptosis. Proc. Natl. Acad. Sci. USA 93: 6737-6742, 1996. PubMed: 8692888 33013: Gibson S, et al. Functional LCK is required for optimal CD28-mediated activation of the TEC family tyrosine kinase EMT/ITK. ...
胞内结构域由共刺激结构域(co-stimulatory domain)和信号转导结构域(signaling domain)构成,其中共刺激域通常来自cd28受体家族(例如cd28和icos)或肿瘤坏死因子受体家族(例如4-1bb、ox40和cd27),信号转导结构域通常为t细胞受体tcr/cd3ζ链,其含有免疫受体酪氨酸活化基序(immunor tyrosine-based activation motifs,itam...
CD28 costimulation, which provides a powerful activation stimulus for Th cell function (16–18). This suggests the potential for an anticancer immunotherapy by the adoptive transfer of TCR gene- modified autologous T cells. MATERIALS AND METHODS Cell Lines. Previously described cell lines used in th...