研究团队首先将酶促脱氨的步骤加入到snmC-seq建库流程中【4】,开发了检测单细胞内5hmC位点的snhmC-seq技术(图2a)。通过系统的优化脱氨反应条件和文库构建的关键步骤,作者提高了检测的灵敏度和稳定性(snhmC-seq2技术),进一步开发出...
Joint-snhmC-seq profiling of single nuclei from mouse brains reveals an unprecedented level of epigenetic heterogeneity of both 5hmC and true 5mC at single-cell resolution. We show that cell-type-specific profiles of 5hmC or true 5mC improve multimodal single-cell data integration, enable ...
(Joint-snhmC-seq), a scalable and quantitative approach that simultaneously profiles 5hmC and true 5mC in single cells by harnessing differential deaminase activity of APOBEC3A toward 5mC and chemically protected 5hmC. Joint-snhmC-seq profiling of single nuclei from mouse brains reveals an ...
scRNA-Seq analysis of human embryonic TMJC cell types To construct a cellular atlas of the human embryonic TMJC in 3 and 4-month-old, we isolated TMJC tissue from two early developmental stages: 7052 cells from 3-month-old TMJC and 9572 cells from 4-month-old TMJC. After cell quality...