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Data were processed using FlowJo v7.6.4. Emission intensities at 585/42 were plotted also as histograms. The 405-nm-excited signal (grey) was normalized by a factor of 1.5-fold for better visualization. The traditional use of the 488 nm wavelength as a mean to detect J-aggregates most ...
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Excitation at 405 nm, like 561 nm, is optimal for flow cytometric analyses of J-aggregates. L1210 cells were incubated with DMSO (control) or 1 μM valinomycin for 30 min at RT and then stained with 2.5 μM JC-1 for additional 15 min. The control and valinomycin treated...