249 -- 1:27 App JC-1 6264 -- 5:19 App 流式细胞实验之调电压 1.6万 3 3:46 App 流式细胞实验之ROS检测(上) 4.8万 8 21:16 App 流式细胞仪详细讲解及数据处理分析 9617 -- 2:48 App 流式细胞实验之ROS检测(下) 1.4万 2 3:08 App ROS检测 4.5万 8 8:23 App FlowJO基本操作 ...
Data were processed by FlowJo v7.6.4. FL2 (585/42) À FL1 (525/50) subtraction (compensation) are shown in percentages. 595 nm (Figure 2a). At 488 nm excitation, the emission intensity at 595 nm was 16-fold higher as would be expected. To permit comparison, we defined the ratio...
实验员小哈&实验系列 - 第二集 - western blot - Day1 - 制胶 17.4万播放 WB实验流程完整视频,赶紧收藏呦 7.8万播放 沉浸式做实验|流式分选&流式细胞图分析+FlowJo圈门操作 9.4万播放 (细胞CCK8实验第二天)细胞不同浓度药物配制给药及6孔板铺板,RAW264.7巨噬细胞 ...
and 405, 488 and 561 nm (LSR-II), and filters: 525/50 and 585/42 nm. Data were processed by FlowJo v7.6.4. Gating strategy is illustrated inSupplementary Figures S1, S2 and S3.
The stained cells were subjected to flow cytometry using a FACSCalibur flow cytometer (BD Biosciences) and data were analyzed with FlowJo software (version 7.6.1; FlowJo LLC). In vivo tumor growth experiment Female BALB/c athymic nude mice (age, 4 weeks; weight, 15-17 g) were obtained ...
Samples were then washed with perm/wash solution (BD biosciences) and fixed in PBS containing 1% formaldehyde before acquisition on BD FACS Celesta flow cytometer (Beckton Dickinson) with BD FACSDiva 8.0.1 software (BD biosciences, Allschwil, Switzerland). All data were analyzed in FlowJo X v...
去上清,加入含2% NCS的PBS 50 μL,依比例加入流式染料,抗体为FITC-AnnexiV和7AAD,冰上放置,抗体染色30min(7AAD最后染5 min)后,离心(4 000 r/min,4 ℃)5 min,沉淀用200 mL含2% NCS的PBS重悬,将细胞悬液转移至流式管,上机检...