159 The PCR technique can detect JC virus DNA in CSF with a reported sensitivity of 75% and a specificity of 96%.160 Serum and CSF antibody titers for JC virus are not useful in establishing the diagnosis of PML
Standard CSF JC virus PCR testing was negative, but ultrasensitive TaqMan real-time JC virus PCR testing was positive, consistent with JC virus-related GCN.Because of the diagnostic challenges in identifying GCN, a high threshold of suspicion should be maintained in patients with an immune-...
PCR for BK virus was performed on plasma samples from: 9 renal-allograft recipients with BK virus nephropathy; 41 recipients without nephropathy (16 of whom had urinary decoy cells), and urine; and 17 subjects with human immunodeficiency virus type 1 (HIV-1) infection who had not undergone ...
At that interaction, a diagnosis of HIV infection was confirmed alongside John Cunningham virus related granule cell neuronopa- thy (JCV-GCN) with JCV PCR positive mildly lympho- cytic cerebrospinal fluid (CSF), in the absence of another detectable cause for her clinic-radiologic presentation. ...
J Frisque Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA Alternative splicing of the JC Virus (JCV) precursor early mRNA yields ve transcripts that encode proteins that regulate the life cycle of this human poly- omavirus. Large...
JC病毒在肾移植受者中的感染状况[1]万方数据
Concentrations of Tat are approxi- mately 1 ng/ml in the serum of HIV-infected patients and 4 ng/ml in the conditioned media of HIV-infected cells (Westendorp et al, 1995; Albini et al, 1998). Interactions between HIV and JC virus JR Berger et al 335 Tat can be readily taken by ...
which is traditionally used to determine virus titer, and Q-PCR values progressively showed the same trend of increase during the course of transfection, although a quantitative discrepancy between the HA titer and Q-PCR data was found, with HA titers remaining low until 14 days p.t. (T14) ...
Pathogenic variants of the J-domain protein DNAJC12 cause parkinsonism, which is associated with a defective interaction of DNAJC12 with tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine biosynthesis. In this work, we characterize the forma
Lentiviral particles containing shRNA targeting Ikaros (Ikzf1gene, Sigma Chemical Co, St. Louis, MO) were produced as described28. Supernatants containing the virus were used to infect RAW 264.7 cells, followed by incubation with puromycin at 2 μg/mL to generate stable lines. Cells containin...