JC virusPosterior fossaProgressive multifocal leukoencephalopathyThe diagnosis of progressive multifocal leukoencephalopathy (PML) is based on a combination of clinical, radiographic, and laboratory findings. However, negative JC polyomavirus (JCPyV) PCR in CSF does not always rule out JCPyV-related PML....
51 A less invasive approach is the detection of virus DNA in the cerebrospinal fluid (CSF) by PCR. CSF parameters usually indicate a mild pleocytosis and slight elevation in protein. Detection of JC virus in the CSF has a sensitivity of 72%–92% and specificity of 92%–100%.85,96 ...
To determine the clinical value of JC virus (JCV) detection in various anatomic compartments for the diagnosis of progressive multifocal leukoencephalopathy (PML).CSF, peripheral blood mononuclear cells (PBMC), plasma, and urine samples were evaluated from HIV-infected and uninfected individuals. JCV ...
After amplification, PCR products were detected by a DNA hybridization method. Microplates were coated with a specific probe and hybridized PCR products were revealed by a commercial colorimetric immunoassay. Using this procedure JC virus DNA was detected in all CSF specimens from patients with ...
Viscount et al.[189]examined whether detection of urinary BK virus by PCR andurine cytologycould identify patients with PVAN. Biopsy-confirmed BK PVAN was diagnosed in four out of 114 patients (3.5%). Using a cutoff value of >1.6E + 04 copies/mL, BKV viremia had a sensitivity and speci...
CSF was positive for JC virus. MRI showed nonspecific abnormal findings. Five days after the clinical debut, the patient died. The clinical picture, MRI imaging, and positive JC virus PCR in CSF strongly suggest ruxolitinib-related JC virus meningitis.Ballesta...
在所述样品上进行实时PCR,其中所述实时PCR引物和探针针对所述JC病毒T抗原。 19.如权利要求18所述的方法,其中所述实时PCR引物和探针的序列分别为SEQ ID NO:1-2和SEQ ID NO:3。 20.一种用于从脑脊髓液(CSF)样品分离核酸的试剂盒,所述试剂盒包括蛋白酶、载体核酸、核酸结合柱以及使用说明书。 21.如权利要求...
His clinical worsening despite the control of his HIV infection was suggestive of IRIS [16], but the increase of the JC virus DNA in the CSF increased despite suppression of the HIV was more consistent with PML progression. When treating IRIS, rapid initiation of systemic steroids has ...
Partial recovery after severe immune reconstitution inflammatory syndrome in a multiple sclerosis patient with progressive multifocal leukoencephalopathy.###Safety, anxiety and natalizumab continuation in JC virus-seropositive MS patients.###High-resolution melting analysis for mutation scanning in the non-cod...
(A) RAW cells and BMMs were stimulated for 20 h with IFNγ and analyzed by qRT-PCR forMCJmRNA levels. The results correspond to the average of 3 independent experiments. *; Student´s t test, p < 0.05. (B) RAW cells were co-transfected with plasmids containing the luciferase ...