JC-1单体(J-monomer)的激发波长为514nm,发射波长为529nm;JC-1聚合物(J-aggregates)的激发波长为585nm,发射波长为590nm。 JC-1可以透过细胞质膜进入细胞,以单体状态游离于胞质内,正常健康细胞的线粒体的膜电位较高,具有极性,JC-1依赖于膜电位的极性被迅速摄入线粒体的基质内,并因浓度增
When excited at 510 nm, JC-1 monomers emit a green fluorescence with a maximum at ~527 nm. Aggregates of JC-1 emit an orange-red fluorescence with a maximum at ~590 nm. 品牌介绍:• MCE (MedChemExpress) 拥有数百种全球独家化合物,我们致力于为全球科研客户提供*新*全的高品质小分子活性...
The technique of JC-1 staining has been developed with the intent to detectDYin intact, viable cells. For this purpose JC-1 acts as a marker of mitochondrial activity, since the formation of J-aggregates, which give red emission, is reversible. Cells with highDYare those forming J-aggregat...
In apoptotic or unhealthy cells, JC-1 exists as monomers which show strong fluorescence intensity with excitation and emission at 485 nm and 535 nm, respectively. The ratio of fluorescence intensity of J-aggregates to fluorescence intensity of monomers can be used as an indicator of cell health...
Monitor the fluorescence intensity. Negative controls with mainly JC-1 J-aggregates can be detected by using an excitation of 540 nm and an emission at 590 nm (Ex/Em = 540/590 nm). Apoptotic or unhealthy cells with mainly JC-1 monomers can be detected at excitation and emission wavelengths...
A drop in ΔΨm, a very early event in apoptosis, results in JC-1 monomers, which fluoresce green (FL1 settings on flow cytometers). JC-1 may also be used with fluorescent microscopes or plate readers, using excitation at 520-570 nm and emission at 570-610 nm for J-aggregates and ...
(Supplementary Figure S1). The near-optimal excitation wavelength for JC-1 monomers is 488 nm; however, monomers, are present throughout the cytoplasmic volume and are thus highly abundant, have significant emission at 585 nm. Therefore, the 585-nm emission from JC-1 in the valinomycin-...
1 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. After cell injury, as membrane potential decreases, JC-1 monomers are generated, resulting in a shift to green emission (529 nm). The principal advantage of JC-1 relative to...
1 dye in the mitochondria and thus, the dye remains in the cytoplasm in its monomer form, leading to a shift from red (agglomerated JC-1, Ex/Em = 585/590 nm) to green fluorescence (JC-1 monomers, Ex/Em = 510/527 nm). JC-1 can be used as an indicator of mitochondrial potential...
While in cells with low ΔΨm, JC-1 remains in the monomeric form. When excited at 510 nm, JC-1 monomers emit a green fluorescence with a maximum at ~527 nm. Aggregates of JC-1 emit an orange-red fluorescence with a maximum at ~590 nm. 品牌介绍:• MCE (MedChemExpress) 拥有数...