While in cells with low ΔΨm, JC-1 remains in the monomeric form. When excited at 490 nm, JC-1 monomers emit a green fluorescence with a maximum at ~520 nm. Aggregates of JC-1 emit an orange-red fluorescence with a maximum at ~590 nm. 品牌介绍:• MCE (MedChemExpress) 拥有数...
JC-1有单体和聚合体两种形式,两者的发射光谱不同。JC-1单体(J-monomer)的激发波长为514nm,发射波长为529nm;JC-1聚合物(J-aggregates)的激发波长为585nm,发射波长为590nm。 JC-1可以透过细胞质膜进入细胞,以单体状态游离于胞质内,正常健康细胞的线粒体的膜电位较高,具有极性,JC-1依赖于膜电位的极性被迅速摄...
When excited at 510 nm, JC-1 monomers emit a green fluorescence with a maximum at ~527 nm. Aggregates of JC-1 emit an orange-red fluorescence with a maximum at ~590 nm. 品牌介绍:• MCE (MedChemExpress) 拥有数百种全球独家化合物,我们致力于为全球科研客户提供*新*全的高品质小分子活性...
4. Fluorescence pattern too much widespread: see point 3.4. Do not consider any event with a very high FL2 fluorescence: very often they are JC-1 aggregates. Increase FSC threshold and discard debris with electronic gating: the presence of stained debris or broken cells can constitute a confo...
JC-1, a fluorescent molecule, is also a probe of P-glycoprotein (Pgp). A specific property of JC-1, due to its stacking in a liquid crystal form, is the dependence of its fluorescence emission wavelength on its concentration. On excitation at 490 nm, JC-1 monomers display a cytoplasmic...
JC-1线粒体膜电位荧光探针 英文名称JC-1 中文名称JC-1线粒体膜电位荧光探针 别名5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide; 保存条件Store at -20℃, protect from l
Monitor the fluorescence intensity. Negative controls with mainly JC-1 J-aggregates can be detected by using an excitation of 540 nm and an emission at 590 nm (Ex/Em = 540/590 nm). Apoptotic or unhealthy cells with mainly JC-1 monomers can be detected at excitation and emission wavelengths...
JC-1 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. After cell injury, as membrane potential decreases, JC-1 monomers are generated, resulting in a shift to green emission (529 nm). The principal advantage of JC-1 relative...
indicated by a green fluorescence emission of 529 nm. At higher concentrations (aqueous solutions above 0.1 µM) or higher potentials, JC-1 dye forms red fluorescent "J-aggregates" where it has accumulated within the mitochondria (Figure...
In healthy cells with a high mitochondrial ΔΨm, JC-1 forms complexes known as J-aggregates, which fluoresce red/orange and may be detected using FL2 settings by flow cytometry. A drop in ΔΨm, a very early event in apoptosis, results in JC-1 monomers, which fluoresce green (FL1 ...