Corning® ITS Premix Universal Culture Supplement, 5mL, 1/Pack (Product #354351) Corning® ITS Premix Universal Culture Supplement, 5mL, contains insulin, human transferrin, and selenous acid, the three most universally essential components of defined culture media. The concentrated supplement stimu...
Corning ITS Premix - Stable for at least three months at 2° to 8°C (lyophilized) or = three months at -20°C (solubilized). Avoid multiple freeze/thaws. Tip Corning ITS Premix - Recommended dilution: 1:1000 (dilute stock solution in serum-free medium) product details at-a-glance ...
采用通用引物ITS-5F/ITS-4R(5’-GGA- AGTAAAAGTCGTAACAAGGPCR-3’/5’-TCCTCC- GCTTATTGATATG-3’)和ITS2F/ITS2R(5’-CGTAA-CAAGGTTTCCGTAGGTGAAC-3’/5’-TTATTGAT-ATGCTTAAACTCAGCGGG-3’)分别扩增ITS和ITS2序列。PCR扩增体系为50 μL:...
ITS(TM) PREMIX 英文别名: ITS(TM) PREMIX 中文名: 中文别名: CBNumber: CB7682586 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商1 ITS(TM) PREMIX化学性质 安全信息 ITS(TM) PREMIX性质、用途与生产工艺 ITS(TM) PREMIX上下游产品信息 ...
HESSE, GERHARD DRPOSPISCHIL, REINER DRSCHNORBACH, HANS-JUERGEN DRSCHUETTE, MANFRED-HEINRICH DRSIRINYAN, KIRKOR DRSONNECK, RAINER DR
PCR扩增体系为50 μL:2×Premix PrimeSTAR HS 25 μL,DNA模板2 μL,正反向引物(10 μmol/L)各1 μL,ddH2O 21 μL。PCR反应条件为:94℃预变性5 min;94℃变性50 s,55℃退火30 s,72℃延伸90 s,35个循环;72℃终延伸10 min。PCR扩增产物经1%琼脂糖凝胶电泳检测合格后,送北京擎科生物科技有限公司进行...
2.2 PCR 扩增及测序 采用通用引物 ITS-5F/ITS-4R ( 5'-GGA- AGTAAAAGTCGTAACAAGGPCR-3'/5'-TCCTCC- GCTTATTGATATG-3')和 ITS2F/ITS2R(5'-CGTAA- CAAGGTTTCCGTAGGTGAAC-3'/5'-TTATTGAT- ATGCTTAAACTCAGCGGG-3')分别扩增 ITS 和 ITS2 序列.PCR 扩增体系为 50 μL:2×Premix PrimeSTAR HS ...
mix blend.gen.premix This will generate ablend/premix.exsfile that needs to be compiled at the top of yourmix.exsfile so that some mix env vars are properly set based on theBLENDenv var before running any mix task. 2. Modify yourmix.exs. ...
结果类似,ITS1 序列远志与卵叶远志之间差异最 PCR 反应在 PTC-200 型 PCR 仪上进行.PCR 总小,仅存在 1 个碱基的差别,其余种间差别都很 体积为 25 μL,包括:12.5 μL Mix(Takara Premix 大;ITS2 序列远志与卵叶远志之间差异最小,仅 Taq),10 μm 引物各 0.5 μL,0.5～1 μL 模板(10～ 存在 1 ...
The sample was reconstituted with H2O-acetonitrile premix and was vortexed for 30 s. All chromatography measurements were conducted using a Waters Alliance HPLC system consisting of a 2694 solvent management system, 2998 photodiode array detector and Empower chromatography workstation. The chromatographic...