The qRT-PCR was performed on a Thermal Cycler Dice Real-Time System III (Takara Bio) using TB Green Premix Ex Taq II (RR820A; Takara Bio). The expression level of each gene was calculated relative to the expression levels of the housekeeping genes β-actin, GAPDH and PSMB6. Primers ...
The Fe2O3/GelMA hybrid hydrogels are not only a scaffold platform for cell culture systems in vitro and tissue remodeling in vivo, but also a potent carrier with magnetic nanoparticles. We provided the detailed recipe in Fe2O3/GelMA hybrid hydrogels (Table S1), and it indicated, in the 20...
Candidatus Izemoplasma, an intermediate in the reductive evolution from Firmicutes to Mollicutes, was proposed to represent a novel class of free-living wall-less bacteria within the phylum Tenericutes. Unfortunately, the paucity of pure cultures has lim
epidermidis), has led to difficulties in the treatment of skin and soft tissue infections (SSTI). The major reason for bacterial resistance is the formation of bacterial biofilm. Here, we report a promising combination therapy of flavaspidic acid BB (BB) and mupirocin, which can effectively ...
The mRNA expression was assessed by qPCR carried out in triplicate by a SYBR Premix Ex Taq™ kit (Takara Bio) and ABI 7900HT Real-Time PCR system (Applied Biosystems Life Technologies, Foster City, CA, USA). For RNA-seq, 1 μg of RNA per sample was used as input material for RNA...
c Mineral premix (each/kg diet): NaCl, 0.1 g; MgSO4·7 H2O, 2.0 g; NaH2PO4·2H2O, 3.3 g; KH2PO4, 4.3 g; Fe-citrate, 0.3 g; ZnSO4·7H2O, 141.2 mg; CoCl2·6H2O, 0.4 mg; KIO3, 1.2 mg; MnSO4·H2O, 64.8 mg; CuSO4·5H2O, 12.4 mg; Na...