Magnetic particle-based DNA extraction methods from blood mainly depend on electrostatic adsorption in a low-pH environment. However, the strong acidic environment can influence the DNA stability. Herein, a polydopamine-functionalized magnetic particle (PDA@Fe3O4)-based protocol was developed for DNA ...
T4 DNA ligaseligation of DNA fragmentNew England Biolabs (NEB) GenJET ™ plasmid miniprepExtraction of plasmid DNA from cultures ofFermentas E. coli GenElute ™ Bacterial GenomicExtraction of genomic DNA from bacteriaSigma-Aldrich DNA(Gram-positive and Gram-negative) Z-Competent ™E. c...
Delta's protocol has the least total number of steps and least amount of total time taken amongst the kits compared. It serves as a universal recipe for both DNA and RNA from pathogens in swab samples including viruses and bacteria in less than 10 min, with competitive performance in both ...