Isomerization of an aspartic acid residue in the complementarity-determining regions of a recombinant antibody to hu- man IgE: identification and effect on binding affinity. Biochemistry. 1996;35(6): 1897-1903.Cacia, J.; Keck, R.; Presta, L.G.; Frenz, J. Isomerization of an aspartic acid...
17.4.2.1 Aspartic acid isomerization Isomerization of aspartic acid residues to isoaspartic acid is a common degradation reaction that impacts protein-based therapeutics. To detect this PTM, a high-resolution mass spectroscopy (MS) method such as MS/MS coupled with peptide map is required. This att...
Isomerization of aspartic acid (Asp) to isoaspartic acid (isoAsp) via succinimide intermediate is a common route of degradation for proteins that can affec... Jennifer,Zhang,Viswanatham,... - 《Methods in Molecular Biology》 被引量: 1发表: 2012年 Competition between(18,18)and(18,16)Config...
Deamidation of asparagine and spontaneous isomerization of aspartic acid in proteins and peptides occur frequently. These modifications result in a mixture... NP Sargaeva,AA Goloborodko,PB O'Connor,... - 《Electrophoresis》 被引量: 15发表: 2011年 Structural basis for dual specificity of yeast N...
The only asparagine residue, Asn108, was deamidated to aspartic acid and isoaspartic acid with a half-life of about 12 days. The same posttranslational modifications were found in recombinant murine full-length protein. On aging, 0.8 mol of isoaspartyl residue per mole of protein was detected ...
The rate of isomerization on heating in vinegar and aqueous AcOH was aspartic acid (Asp)>serine(Ser)proline (Pro), whereas in 6M HCl it was Asp>ProSer. Heating of L -Pro together with glucose and fructose in 5% AcOH at 100°C for 96h led to the formation of 24% D -Pro relative ...
The only asparagine residue, Asn108, was deamidated to aspartic acid and isoaspartic acid with a half-life of about 12 days. The same posttranslational modifications were found in recombinant murine full-length protein. On aging, 0.8 mol of isoaspartyl residue per mole of protein was detected ...
(Supplementary Methods1.3.1), ultimately, in the presence of 5 mol% Pd(OAc)2and 10 mol%N-Cbz-aspartic acid (Cbz-Asp-OH) as a ligand, and one equivalent of Ag2CO3as the halide scavenger, after 24 h reaction at room temperature (25 °C) in ethanol under air, the desired...
Asp23Asnmimics the structures of the native and isomerized segments. We modeled an L-Asn residue at position 23 of the Aβ20–34, isoAsp23structure to see if the native L-amino acid could be accommodated in the dry L-isoAsp interface B (Fig.4c, right panel). The L-Asn residue was ...
Wakankar AA, Liu J, Vandervelde D, Wang YJ, Shire SJ, Borchardt RT. The effect of cosolutes on the isomerization of aspartic acid residues and conformational stability in a monoclonal antibody. J Pharm Sci. 2007; 96 (7):1708–1718....