Bacterial artificial chromosome cloning\n Escherichia coli–streptomycete artificial chromosomeIsolation of high molecular weight DNA fragments from soil, in excess of 1 Mb, and of sufficient quality for cloning into an Escherichia coli streptomycete artificial chromosome vector is described. The ...
Isolation of DNA from bacterial samples of the human gastrointestinal tract. Nat Protoc 2006;1:870e3.Zoetendal EG, Heilig HGHJ, Klaassens ES, Booijnk CCGM, Kleere- bezem M, Smidt H, et al, Isolation of DNA from bacterial samples of the human gastrointestinal tract. Nat Protoc 1:870-873...
DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community We developed a protocol which yields purified bacterial DNA from the soil bacterial community. The bacteria were first dispersed and separated from soil pa... William,E.,Holben,... - 《Applied & Envi...
The currently known methods of DNA isolation from soil samples are usually laborious and multistage. In our opinion, a promising approach to solve this problem could be based on the use of a two-component bioseparating element. The first component of such element is a special sorbent ...
This protocol for DNA isolation from forest soil samples is advantageous because it uses only one liquid transference step and can process several samples
Comparison of methods for isolation of bacterial and fungal DNA from human blood. Curr Microbiol, 2014; 68, 149-55.Gosiewski T, Szała L, Pietrzyk A, Brzychczy-Włoch M, Heczko PB, Bulanda M: Comparison of methods for isolation of bacterial and fungal DNA from human blood. Curr ...
a total of 140 bacterial strains were isolated from 66 soil samples.By sequencing analysis of 16 S r DNAs,66 bacterial species belonging to 22 genera were identified,of which 69 strains of siderophore-producing bacteria were screened,belonging to 35 species of 14 genera,among which the dominant...
Disruption of Firmicutes and Actinobacteria abundance in tomato rhizosphere causes the incidence of bacterial wilt disease ArticleOpen access07 October 2020 Introduction The rhizosphere is the portion of soil that is directly influenced by the plant roots, encompassing root surfaces as well as the strongl...
Preparation of bacterial DNA samples Bacterial genomic DNA was isolated from buccal mucosal swabs or tissues using a commercial kit for soil bacteria (MOBIO Laboratories, Carlsbad, CA, USA). The tissues were pretreated before DNA extraction as previously described11. Briefly, the tissues were washed...
The present study was conducted with a view to isolate and characterize endophytic bacterial diversity from endorhizosphere of sugarcane (sp.) and rye grass (). Eight bacterial isolates from sugarcane and seven from rye grass were obtained and identified as and . S1 and R1 (sugarcane and ryegras...