DnaT is one of the replication restart primosomal proteins required for reinitiating chromosomal DNA replication in bacteria. In this study, we identified and characterized the single-stranded DNA (ssDNA)-binding properties of DnaT using electrophoretic mobility shift analysis (EMSA), bioinformatic tools...
DURING a systematic survey of the substrate specificities of a variety of restriction endonucleases, it was discovered that the Haemophilus aegyptius restriction endonuclease III (HaeIII) specifically cleaved ‘single-stranded’ DNA from φX174 and M13. Since these DNAs are not double-stranded1 and ...
What would be the ramifications if DNA was single stranded? Where does the DNA in CODIS come from? Who discovered DNA microarrays? Who found double-stranded RNA? How is single-stranded DNA spliced? Are there single-stranded DNA molecules?
The ATR-CHK1 DNA damage response pathway becomes activated by the exposure of RPA-coated single-stranded DNA (ssDNA) that forms as an intermediate during DNA damage and repair, and as a part of the replication stress response. Here, we identify ZNF827 as a component of the ATR-CHK1 kinase...
'Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases. Nature. 1975 Oct 2; 257 (5525):421–422.Blakesley, R. W.; Wells, R. D. `Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases. Nature 1975, 257, 421-422....
in a proteomics screen for novel meiotic chromatin-associated proteins in mice. MEIOB contains an OB domain with homology to one of the RPA1 OB folds. MEIOB binds to single-stranded DNA and exhibits 3′–5′ exonuclease activity. MEIOB forms a complex with RPA and with SPATA22, and these ...
How is methylated DNA replicated? What is a spotted DNA microarray? How is DNA methylation used in DNA repair? What happens if DNA repair genes are damaged? Is there DNA in your food? How do you know? Why is single-stranded DNA unstable?
This is a cycle with common steps: DNA base synthesis on single stranded DNA, followed by detection of the incorporated base, and then subsequent removal of reactants to restart the cycle. Most sequencing instruments use optical detection...
During PCR and cycle sequencing, the DNA is first denatured (the double-stranded DNA template becomes single-stranded DNA). A subsequent annealing step allows for hybridization of the oligonucleotide primer close to the sequence of interest. In the extension...
In vitro, this requires single-stranded DNA (ssDNA) substrates and is strand specific: only the “top” strand is recognized by the element-encoded transposase, TnpA, and is cleaved and transferred, whereas the “bottom” strand does not transpose. Excision of the top strand as a transposon ...