Activation of the T-DNA transfer process of Agrobacterium by the plant signal molecule acetosyringone generates a single-stranded, unipolar, linear T-DNA molecule (T-strand)—a potential conjugative intermediate in the transfer of the T-DNA to plant cells. Acetosyringone induction also leads to ...
Which molecule is always single-stranded? RNA is always single-stranded and is often referred to as single-stranded ribonucleic acid. This can be seen in Figure 1 below. However, DNA can be either double-stranded or a single strand of DNA (ssDNA).Figure 1. The comparison between single-...
form a single repeat. After annealing is complete, any remaining single stranded nonhomologous flaps (remnants from the 3′ overhangs) are reselected by Rad1 andERCC1. Lastly,DNA synthesisfills in any gaps and ligation produces a continuous double stranded DNA molecule and DSB repair is complete...
约500 000个DNA功能模块的变种(单核苷酸多 态性 ,SNP,它们遍布DNA的整个长度,并被证明与某些生理和行为特征或疾病敏感性相关)正在接受常规分析。 project-syndicate.org [...] approximately 700 nm × 11 nm, and are composed of a single-stranded RNA molecule consisting of almost 10 000 nucleotides...
DNA deoxyribonucleic acid: an extremely long, double-stranded nucleic acid molecule arranged as a double helix that is the main constituent of the chromosome and that carries the genes as segments along its strands: found chiefly in the chromatin of cells and in many viruses. ...
M. Simultaneous, coincident optical trapping and single-molecule fluorescence. Nat. Methods 1, 133–139 (2004). Article Google Scholar Lee, K. S., Balci, H., Jia, H., Lohman, T. M. & Ha, T. Direct imaging of single UvrD helicase dynamics on long single-stranded DNA. Nat. Commun...
This paper aims at a comprehensive understanding on the novel elastic property of double-stranded DNA (dsDNA) discovered very recently through single-molecule manipulation techniques. A general elastic model for double-stranded biopolymers is proposed and a new structural parameter called the folding angl...
(Science 271:795–799, 1996) in which an individual double-stranded DNA (dsDNA) molecule can be captured between two polystyrene beads. The first bead is immobilized by the optical tweezers and the second by the micropipette. Movement of the micropipette allows manipulation and stretching of the...
The ATR-CHK1 DNA damage response pathway becomes activated by the exposure of RPA-coated single-stranded DNA (ssDNA) that forms as an intermediate during DNA damage and repair, and as a part of the replication stress response. Here, we identify ZNF827 as a component of the ATR-CHK1 kinase...
Aptamers are short single-stranded oligonucleotides (DNA or RNA, typically 20–100 base pairs) whose sequence is able to specifically bind target molecule(s) with very high affinities, equivalent to those of antibodies. They can be generated against various targets such as divalent metal ions, ...