A cortical granule-free domain (CGFD) overlies the metaphase chromatin in fully mature mouse eggs. Although a chromatin-induced localized release of cortical granules (CG) during maturation is thought to be a major contributing factor to its formation, there are indications that CG redistribution ma...
even if they are dissociated from chromatin, might be sufficient to transfer memory of the gene transcription program21. Nevertheless, it is widely believed that chromatin-based mechanisms provide a means to regulate gene expression during cell division. In this scenario, although many transcription fa...
Liquid–liquid phase separation (LLPS) is an important mechanism that helps explain the membraneless compartmentalization of the nucleus. Because chromatin compaction and LLPS are collective phenomena, linking their modulation to the physicochemical features of nucleosomes is challenging. Here, we develop ...
How do chromosomes behave in prophase 1 and metaphase 1 and how does this allow variation to take place? What is main difference between monohybrid and dihybrid cross? What is the difference between chromatin and histone? Explain the difference between chromatins and chromatid...
Next, we examined the effect of OS on DNA 5mC and 5-hmC levels using immunofluorescence in germinal vesicle (GV) and metaphase II (MII) oocytes (Fig. 1A-D). Global DNA methylation levels were remarkably decreased, and DNA 5-hmC levels were significantly increased in OS GV oocytes compared...
Chiasmata are specialized chromatin structures that link homologous chromosomes together until anaphase I (Figs. 45.1 and 45.10). They form at sites where
Heterochromatin protein 1α (HP1α) is involved in regulation of chromatin plasticity, DNA damage repair, and centromere dynamics. HP1α detects histone dimethylation and trimethylation of Lys-9 via its chromodomain. HP1α localizes to heterochromatin in interphase cells but is liberated from ...
The SMC complexes play a crucial role in the chromosome partitioning process by organizing bacterial chromosomes through their ATP-dependent chromatin-compacting activity. Recent progress in the composition of these complexes and elucidation of their structural and enzymatic properties has advanced our ...
There is a strong demand for methods that can efficiently reconstruct valid super-resolution intact genome 3D structures from sparse and noise single-cell Hi-C data. Here, we develop Single-Cell Chromosome Conformation Calculator (Si-C) within the Bayesi
After 24 h, cells were arrested in metaphase stage of mitosis by using colcemid (10 μg/ml, 15210-016, KaryoMAX) for 12 h. The cells were then harvested and fixed in methanol: acetic acid (3:1). Metaphase spreads were stained with Giemsa and visualized under the microscope. ...