Osteosarcoma (OS) remains a dismal malignancy in children and young adults, with poor outcome for metastatic and recurrent disease. Immunotherapies in OS are not as promising as in some other cancer types due to intra-tumor heterogeneity and considerable
Comparison with the sequence of the wild-type gene copy confirmed a point mutation in the first position of the codon at amino acid position 627 from C to T resulting in a TGA stop codon instead of CGA for arginine. The truncated CPR peptide is, therefore, 83 amino acids shorter than ...
This line was previously characterized as a cytosolic ascorbate peroxidase 1 (APX1) mutant24,36 and contains a T-DNA insertion in the seventh intron of the gene At1g07890, 160-bp upstream of the stop codon. The genetic study in Arabidopsis was completed with the characterization of the ...
1 A similar pattern of m6A distribution was observed that these m6A peaks were enriched in coding sequence and 3′ UTR with a sharp density peak surrounding the stop codon (Figure 4C). METTL3 knockdown led to reduced number of m6A peaks (Figure 4C) and m6A abundance of common peaks (...
Species phylogeny was inferred using RAxML (PROTCATWAG model) with 1,000 bootstrap replicates; all nodes were supported by at least 99 % of replicates. Ortholog conservation (right panel) highlights genes conserved in all species (core, green), genes conserved in at least two species (...
Enzymatic analyses of Bt extracts suggested that a modified TCA operates during the sporulation phase of growth in conjunction with the glyoxylic acid cycle and the GABA pathway [6]. The Bt gab gene cluster is involved in GABA degradation. Previously, we proposed a model to explain the ...
Translated GXY repeats within the collagen-like (CL) region are shown with an asterisk representing the location of the premature stop codon resulting in a truncated protein. V, variable region; L, linker region; WM, wall-membrane associated region. Below, spectrophotometric measurements of 24-h ...
nuclear pores (14), HSV-1 evolved a mechanism for transport- ing nucleocapsids across the nuclear membrane (NM). Thus, progeny HSV-1 nucleocapsids acquire primary envelopes by budding through the inner NM into the space between the inner and outer NMs, the perinuclear space (30, 48). Altho...
A deletion of WNV NS1 affects viral RNA synthesis and formation of the replication complex. A-B. Measurement of negative (A) or positive (B) strand viral RNA in BHK21-VEEV-NS1 or BHK21-VEEV-NS1-IS (premature stop codon) cells infected with WNV-WT or ∆-NS1-WNV. The indicated cells...
However, a protein of 85 amino acid residues (Fig. 3B) could be potentially translated from an AUA codon which was shown to be recognized in some cases as an initiation codon in organelles of eukaryotes (Folley & Fox, 1991 ; Mulero & Fox, 1994 ; Nickelsen et al., 1999). ' Stop-to...