How much is TAQ PLUS enzyme - chemical and physical properties, structure, melting point, boiling point, density, molecular formula, molecular weight, uses, prices, suppliers, and toxicity/safety/hazards/SDS information.
Step-by-Step Solution:1. Understanding the Question: The question asks about the source of Taq polymerase, a specific enzyme used in biotechnology, particularly in PCR (Polymerase Chain Reaction).2. <str
How did Taq DNA polymerase acquire its name? What is the purpose of DNA helicase? How does DNA polymerase interact with DNA? Is DNA polymerase an enzyme? How is PCR used in DNA sequencing? Is DNA polymerase a protein? Why is DNA polymerase a required part of PCR? a. It synthesizes pr...
and Murthy, H.M.: Crystal structure of Taq DNA polymerase in complex with an inhibitory Fab: The Fab is directed against an intermediate in the helix-coil dynamics of the enzyme, Proc. Natl. Acad. Sci. U.S.A., Vol.95, No.21, pp.12562-12567 (1998)....
We next performed a gap-filling assay with another Taq DNA polymerase, which lacks 3’->5’ nuclease activity. The mutation frequency in Taq + MIF-WT group was about 7 mutants/104 clones, which was much lower than those (14–22 mutants/104 clones) observed in the nuclease-inactive E22A...
To express thebilRSgenes fromClostridium symbiosuminE. coli, the gene was amplified and inserted into the expression vector backbone pCW–lic (Addgene, 26908). A polymerase chain reaction (PCR) amplification using OneTaq Master Mix (New England Biolabs (NEB), M0482) was performed onClostridium ...
By using Taq DNA polymerase (Roche Molecular Biochemicals, Indianapolis, IN) and the protocol recommended by the supplier, a 2.5-kb fragment including the idi gene was amplified. The PCR fragment was digested with HindIII and cloned into the multi-cloning site of an E. coli vector pUC118 to...
AccuTaq LA DNA polymerase (Sigma, USA) was used for PCR in accordance with the manufacturer's instructions. 2.3. Shotgun experiment Total genomic DNA from B. amyloliquefaciens A50 (10 μg) was digested with PstI, ligated with the similarly digested vector pCB22 (10 μg) and transformed ...
libraries were purified using AMPure XP beads at a 1.8X bead to sample ratio. A first extension was completed with an enzyme mix from Exact Sciences Innovation before library amplification was performed using KAPA HiFi HotStart Uracil+ ReadyMix Kit (KAPA Biosystems), according to the manufacturer’...
(Sigma, USA). The resulting gDNA was diluted 10x and 1 μl was added to our PCR master mix (Taq DNA polymerase [1U/ml; Life Technologies 10342020], 1x Taq Buffer [Life Technologies], 0.2 µM primer mix [FW, RV + M13], 0.2 µM dNTPs, 1.5 mM MgCl2) to a ...