Step-by-Step Solution:1. Understanding the Question: The question asks about the source of Taq polymerase, a specific enzyme used in biotechnology, particularly in PCR (Polymerase Chain Reaction).2. <str
Polymerase chain reaction (PCR) is a widely used technique to produce large numbers of DNA molecules in a short amount of time. The ideal DNA polymerase enzyme for PCR is the Taq polymerase.Answer and Explanation: Most enzymes are prone to degradation at high temperatures. However...
A previously reported directed enzyme evolution experiment by in vitro selection of Stoffel fragment variants of Taq DNA polymerase I was used here to predict the activities of Streptococcus agalactiae DNA polymerase I. The reverse transcriptase activity of S. agalactiae DNA polymerase I was measured ...
Applied Biosystems' TaqMan qPCR is based on the most important enzyme in PCR, Taq polymerase. With an average workflow time of just two hours, Applied Biosystems' TaqMan qPCR assays exploit the 5' nuclease activity of Taq Polymerase and fluorescent hydrolysis probes to increase...
These lines of evidence provide definitive evidence that an enzyme distinct from the mRNA poly(A) polymerase is involved in the adenylation of SRP RNA. S. cerevisiae genome is known to contain a single copy of the poly(A) polymerase (12). In the yeast pap mutant strains that we used in...
We next performed a gap-filling assay with another Taq DNA polymerase, which lacks 3’->5’ nuclease activity. The mutation frequency in Taq + MIF-WT group was about 7 mutants/104 clones, which was much lower than those (14–22 mutants/104 clones) observed in the nuclease-inactive E22A...
To express thebilRSgenes fromClostridium symbiosuminE. coli, the gene was amplified and inserted into the expression vector backbone pCW–lic (Addgene, 26908). A polymerase chain reaction (PCR) amplification using OneTaq Master Mix (New England Biolabs (NEB), M0482) was performed onClostridium ...
Conclusion Altogether, this study provides an in-depth genomic por- trait of a fungal castrating, biotrophic plant pathogen reflecting its unique life cycle. In particular, the unique absence of enzyme classes for plant cell wall degradation and maintenance of enzymes that break down compo- nents ...
Each 50μL PCR reaction contained 20–40 ng genomic DNA, 1x PCR buffer, 10 mM dNTP mix, 5uM of each primer and 1 U Hot Start Taq polymerase. PCR was performed in Mastercycler PCR machine (Eppendorf) using a hot start cycling profile, which starts with an initial denaturation at 96 ...
Each PCR reaction (25 μl) consisted of 0.625 unit of Go-Taq DNA polymerase, 1.5 mM MgCl2, 0.2 mM each of dNTP, 0.2 μm each primer, 1 × Colorless Go-Taq Reaction buffer (Promega). PCR was run under the following conditions: 1 cycle of 95°C, 2 min; 33 cycles of 95°C, 30...