Taq DNA聚合酶在长片段扩增上，最卓越的成果是Klentaq1与Pfu混合酶（Barnes, 1994; U.S. Patent 5436149），可以扩增长达35kb的Lambda DNA片段，保真性也比Taq DNA聚合酶显著提高，但单独的Klentaq1持续合成能力是显著下降的（比Taq低10倍）。Ignatov等（US 11/658,610）将Tth DNA polymerase（该酶的扩增能力高...
Taq DNA Polymerase价格:￥550/支产品详情:概述(Summary)icon储存条件(Storage)储存于 -20°C,保质期 12 个月状态(Form)Liquid储存溶液(Buffer)20mM Tris-HCl (pH... 纯度:>99% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining. ...
Meridian’s Taq Dilution Buffer is an optimized buffer that has been designed to conferring long-term stability to high-quality Taq DNA polymerase.
Anti-polA/Taq polymerase 1 Antibody(单克隆抗体) 价格电议 起批量≥1 支 最小起订1支 供货总量99999支 发货地址湖北省武汉市 建议售价￥2300/支 更新日期2025年04月16日 产品规格100ug 即时洽谈立即询价查看联系方式 收藏产品发送留言 VIP3年 武汉佰乐博生物技术有限公司 ...
Taq-Polymerase You have full access to this open access chapter,Download reference work entry PDF Literatur Mullis KB, Ferré F, Gibbs RA (Hrsg) (1994) The polymerase chain reaction (PCR). Birkhäuser Verlag AG, Basel Google Scholar
5.Taq DNA Polymerase. Usually 1-1.5u of Taq DNA Polymerase are used in 50µl of reaction mix. Higher Taq DNA Polymerase concentrations may cause synthesis of nonspecific products. However, if inhibitors are present in the reaction mix (e.g., if the template DNA used is not highly purifi...
The enzyme is a full-length form of Taq DNA polymerase that exhibits 5´→3´ exonuclease activity. GoTaq® G2 DNA Polymerase is supplied with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. Both buffers contain MgCl2 at a concentration of 7.5mM for a ...
Usually 1-1.5u ofTaqDNA Polymerase are used in 50µl of reaction mix. HigherTaqDNA Polymerase concentrations may cause synthesis of nonspecific products. However, if inhibitors are present in the reaction mix (e.g., if the template DNA used is not highly purified), higher amounts ofTaqDNA...
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form. General PCR protocol The following procedure is presented as general guideline for using AccuStart II Taq DNA Polymerase in any PCR procedure. Cycling conditions, concentration of, primers, ...
MyTaq HS DNA Polymerase 1 x 50 l 2 x 250 l 1 x 200 l 5x MyTaq Reaction Buffer 2 x 1ml 14 x 1.5ml 8 x 1ml Step Temperature Time Cycles Initial denaturation 95°C 1min 1 Denaturation 95°C 15s 25-35 Annealing* User determined ...