Taq DNA聚合酶在长片段扩增上，最卓越的成果是Klentaq1与Pfu混合酶（Barnes, 1994; U.S. Patent 5436149），可以扩增长达35kb的Lambda DNA片段，保真性也比Taq DNA聚合酶显著提高，但单独的Klentaq1持续合成能力是显著下降的（比Taq低10倍）。Ignatov等（US 11/658,61
AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.
状态(Form) Liquid 储存溶液(Buffer) 20mM Tris-HCl (pH8.0)、0.1 mM EDTA、1mM DTT、100 mM KCl、 Stabilizers、50% glycerol 表达宿主(Host) E.coli 分子量 94kDa 纯度 >99% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining. 酶活单位定义 在74℃、30分钟内,以活性化的...
Die Aktivität der Taq-Polymerase wird in Units angegeben und 1 Unit als die Enzymmenge definiert, die in 30 Minuten bei 72 °C 10 nmol dNTP in eine säureunlösliche Form überführt. Literatur Mullis KB, Ferré F, Gibbs RA (Hrsg) (1994) The polymerase chain reaction (PCR). Bir...
Anti-polA/Taq polymerase 1 Antibody(单克隆抗体) 价格电议 起批量≥1 支 最小起订1支 供货总量99999支 发货地址湖北省武汉市 建议售价￥2300/支 更新日期2025年05月12日 产品规格100ug 即时洽谈立即询价查看联系方式 收藏产品发送留言 VIP3年 武汉佰乐博生物技术有限公司 ...
Meridian’s Taq Dilution Buffer is an optimized buffer that has been designed to conferring long-term stability to high-quality Taq DNA polymerase.
5.Taq DNA Polymerase. Usually 1-1.5u of Taq DNA Polymerase are used in 50µl of reaction mix. Higher Taq DNA Polymerase concentrations may cause synthesis of nonspecific products. However, if inhibitors are present in the reaction mix (e.g., if the template DNA used is not highly purifi...
Usually 1-1.5u ofTaqDNA Polymerase are used in 50µl of reaction mix. HigherTaqDNA Polymerase concentrations may cause synthesis of nonspecific products. However, if inhibitors are present in the reaction mix (e.g., if the template DNA used is not highly purified), higher amounts ofTaqDNA...
The enzyme is a full-length form of Taq DNA polymerase that exhibits 5´→3´ exonuclease activity. GoTaq® G2 DNA Polymerase is supplied with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. Both buffers contain MgCl2 at a ...
Full size image This broad peak may represent the FAM with a T nucleotide (FAM-5′-T-3′). It is possible that at elevated temperatures, two strands of DNA locally melt at the terminus and form a structure recognized and cleaved by the Taq polymerase with its flap-end nuclease activity21...