These data suggest that zygotic transcription is activated during the early cleavages of Helobdella embryos and that newly synthesized transcripts are required for the generation of teloblasts. Thus, there is an early, critical period of messenger RNA synthesis essential for teloblast production that ...
百度试题 题目In bacteria, transcription and translation are tightly coupled. RNA synthesis is semiconservative.反转录酶具有RNase活性。相关知识点: 试题来源: 解析 错误 反馈 收藏
Transcription, GeneticThe photomicrographs of 'genes in action' taken by Miller and colleagues strikingly illustrate transcription of eukaryotic genes by RNA polymerase moving along the DNA 1,2 . Although completed transcripts may be associated with sub-nuclear structures 3–7 , the view that a ...
2. RNA library preparation: the extracted RNA is reverse transcribed, cDNA is synthesized, and adapter sequences are added to it to form an RNA-seq library. 3. Sequencing: RNA-seq libraries are sequenced at high throughput, usually using sequencing platforms such as Illumina or Ion Torrent. ...
100 ng of total RNA, isolated from HAECs using a glass filter based kit, was amplified using Ambion's MessageAmp™ aRNA Kit, producing 4-5 µg of aRNA (cRNA). 2 µg of this aRNA was labeled with 33P by reverse transcription using hexanucleotide...
Transcription-coupled repair in yeast is independent from ubiquitylation of RNA pol II... 来自 EBSCO 喜欢 0 阅读量: 61 作者:Lommel,Lori,Bucheli,Miriam,E.摘要: Demonstrates that removal, repression or over-expression of Rsp5 has no effect on transcription-coupled nucleotide excision repair (TCR)...
4. The well-known potential source of this cell-to-cell variability is a noisy messenger RNA production in transcription, which is the so-called ‘transcriptional bursting’, and this mechanism has been broadly studied by theoretical and experimental approaches. According to the theoretical studies,...
Total RNA was isolated and DNase I-treated using the RNeasy Kit (Qiagen). The cDNAs were synthesized by Moloney murine leukemia virus RT with random primers (Invitrogen), and analyzed by quantitative PCR with SYBRTM Green in triplicate on a Bio-Rad CFX thermal cycler. The RT-qPCR products...
Sense and antisense digoxigenin-labeled probes were synthesized in vitro using T7 and T3 polymerases, respectively, and a proprietary kit (MEGAscript®; Ambion, Inc., Austin, TX) supplemented with DIG RNA labeling mix (Roche Applied Science) and RNAguardTM (GE Healthcare). Labeled RNA probes...
Given the recent discovery of widespread pervasive transcription in all forms of life, protection of neighboring genes from intergenic transcriptional interference may be a key extratranscriptional function of assembled RNA polymerase III complexes and possibly other DNA binding proteins. 展开 关键词: RNA...