Transcription is the process in which the nucleotide sequence of a gene is used as a template to direct the synthesis of RNA. (It takes place in the nucleus) firstly the double-stranded DNA unzips, and the mRNA strand produced (the sense transcript) will be complementary to the original ...
Why does RNA transcription occur?Gene expression:Gene expression is the process by which the information within a gene is accessed for transcription into mRNA molecules that in turn are translated into polypeptide chains. These polypeptide chains fold into functional proteins that determine an organism'...
RNA Polymerase IITranscription Factors, TFIIWhen transcription by RNA polymerase II from the major-late (ML) promoter was studied with purified basal transcription factors, it was observed that transcription from negatively-supercoiled ML templates did not require transcription factor IIH (TFIIH). ...
CRISPR RNA (crRNA) and Cas9:CRISPR DNA serves as a permanent record of past infections, but for bacteria to use these sequences to thwart viruses, they must convert them into DNA's cousin,RNA. Through a process called transcription, bacteria first copy one of the two CRISPR DNA strands int...
Before delving into the mechanics of DNA transcription, it's worth taking a moment to review the terminology associated with DNA and RNA, because with so many similar-sounding words in the mix, it can be easy to confuse them. Replicationis the act of making an identical copy of something....
2.4. Reverse-Transcription PCR Total RNA was extracted using TRIzol (Catalog 15596018, Life Technologies, Carlsbad, CA, USA). A total of 10 μg of total RNA was incubated with 10 U RNase-free DNase I (Catalog EN0521, Life Technologies) at 37 °C for 30 min. RNA was further purified ...
At the promotion stage, inflammatory cytokines (notably TNF, IL-1β and IL-6) induce NF-κB and STAT3 transcription factors in cancer cells, which activate genes controlling cell survival, proliferation and growth. Inflammatory cytokines are produced by cancer cells themselves or by immune cells ...
Rapid quantification of human ABCA1 mRNA in various cell types and tissues by real-time reverse transcription-PCR Oligo(dT)-immobilized PP microplates (GenePlate-PP, AGCT), Yoyo-1 (Molecular Probes), reagents for PCR [Taq polymerase and EZ rTth RNA-PCR kit (Perkin-E1mer)], the K562 cell...
RNA structure maps across mammalian cellular compartments Nat. Struct. Mol. Biol., 26 (2019), pp. 322-330 CrossrefView in ScopusGoogle Scholar 40 A. Ray-Soni, M.J. Bellecourt, R. Landick Mechanisms of Bacterial Transcription Termination: All Good Things Must End Annu. Rev. Biochem., 85...
Nidovirus transcription: how to make sense...? Sense-antisense base-pairing interactions between short conserved sequences play a key regulatory role in this process. In view of the presumed common ... Pasternak,O A. - 《Journal of General Virology》 被引量: 580发表: 2006年 ...