The protease mu-calpain was purified from bovine skeletal muscle and used to digest at-death MF at pH 5.6, 4 degrees C, 100 microM CaCl2. Degradation of the proteins titin, nebulin, filamin, desmin, and troponin-T was monitored in the PM and mu-calpain-digested samples by using SDS-...
A NOVEL BRAIN CYSTEINE PROTEASE IS CAPABLE OF PROCESSING THE B AMYLOID PRECURSOR PROTEINdoi:10.1097/00005072-199505000-00023Chang, TienAbraham, Carmela RJournal of Neuropathology & Experimental Neurology
LC3/ATG8 has long been appreciated to play a central role in autophagy, by which a variety of cytoplasmic materials are delivered to lysosomes and eventually degraded. However, information on the molecular functions of LC3 in RNA biology is very limited.
GPR37L1 is an orphan G protein-coupled receptor expressed exclusively in the brain and linked to seizures, neuroprotection and cardiovascular disease. Based upon the observation that fragments of the GPR37L1 N-terminus are found in human cerebrospinal fl
Transfected HEK293T cells were washed with PBS and scraped into NP-40 lysis buffer (25 mM Tris HCL [pH 7.5], 50 mM NaCl, 1% NP-40, 2 mM EDTA, and cocktail of protease inhibitors). After 15 min on ice, the cells were homogenized in chilled racks with a TissueLyser bead mill (Invit...
摘要: BackgroundParaneoplastic pemphigus (PNP) is a devastating autoimmune blistering disease, involving mucocutaneous and internal organs, and associated with underlying neoplasms. PNP is characteri ...关键词: G protein-coupled receptor GPCR phosphoproteome ...
Due to the complexity of protein biochemistry, it is challenging to predict the optimal surfactant to extract a given protein. Thus, experimenting with different surfactants is recommended if you encounter issues. This is especially recommended for membrane proteins. 4. Protease inhibitors Tissues and ...
QIAGEN 1020952 Protease Resuspension Buffer (6/15),KG QIAGEN 1019266 QIAquick 96 PB Buffer Module 8000 QIAGEN 1054575 HiSpeed Plasmid Giga EF Kit (5) QIAGEN 1061072 AIV-H5 RT-PCR REAGENT QIAGEN 1061094 HCV RT-PCR REAGENT VII QIAGEN 1061115 PG FMDV RT-PCR Assay (48) Z ...
protein abundance between conditions (Supplementary Data4). After normalization, we identified 670 phosphopeptides corresponding to 399 phosphoproteins with a ≥2.0 or ≤−2.0 -fold difference in the abundance between Pru and PruΔpp2a-c, and 1076 differential phosphopeptides on 485 proteins between ...
Protein isolation and western blotting Placental tissue was homogenised in protein lysis buffer (50 mmol/l Tris pH 8.0, 150 mmol/l NaCl, 0.5% deoxycholate, 1% Triton X-100, 0.1% [wt/vol.] sodium dodecyl sulphate [SDS]) plus 2 mmol/l phenylmethylsulfonyl fluoride (PMSF), a protease (Roc...