The colonies were cultured in 50-mL tubes with 10 mL of YEPS medium (10 g/L yeast extract, 20 g/L peptone, 10 g/L sorbitol, and 100 mM potassium phosphate buffer pH 6.0). Cultures were incubated at 28 °C and 250 rpm and 5 g/L methanol were added daily as ...
State as to why (a) a solution ofNa2CO3is alkaline ? (b) alkali metals are prepared by electrolysis of their fused chlorides? (c) sodium is found to be more useful than potassium ? View Solution Exams IIT JEE NEET UP Board Bihar Board ...
Potassium peroxymonosulfate has a high potential value of 1.85v, surpassing most oxidants such as chlorine dioxide, potassium permanganate, sodium hypochlorite, and hydrogen peroxide in terms of oxidation ability. This potential value can oxidize most organic matter, reduce oxygen consumption factors, ...
Briefly, 1 μmol of NAADP was incubated with 100 μl of 100 mM sodium periodate/100 mM sodium acetate (pH 5.0) mixture in the dark for 1 h. The unreacted sodium periodate was removed by adding 200 mM potassium chloride to the solution, incubating on ice for 5 min, ...
After washing with PBS 3 times, the slides were incubated with freshly prepared SA-β-Gal activity detection solution (1 mg/ml X-gal, 5 mM Potassium Ferrocyanide, 5 mM Potassium Ferricyanide, 2 mM MgCl2, pH to 6.0) at 37 °C overnight. After the incubation, the tissues were countered ...
A potassium cyanide (KCN) solution (100 µM, Sigma Aldrich) was mixed with Hb (50 µM) prior to the experiment for 1 h to allow complete complexation of the heme iron to prevent any oxidation reaction. The complex was subsequently incubated with pDNA (25 µg/ml) at 37 °C for...
, after bicarbonate infusion increased generated echogenicity 16-21 dB higher than for pristine urine or saline infusion for the same peak rarefactional pr... EY Hwang,JB Fowlkes,PL Carson - Ultrasonics Symposium 被引量: 4发表: 1999年 Effect of dietary sodium and potassium on heart, small an...
Reaction with sodium acetate, for example, displaces acetic acid:H2SO4 + CH3COONa → NaHSO4 + CH3COOHSimilarly, reacting sulfuric acid with potassium nitrate can be used to produce nitric acid and a precipitate of potassium bisulfate. When combined with nitric acid, sulfuric acid acts both as ...
Cells were harvested with cold 50 mM potassium phosphate buffer (pH 7.4) containing 1 mM EDTA and then sonicated. Homogenized cells were centrifuged at 10,000 x g for 15 min at 4 °C and the supernatant was added to the wells of a 96-well plate, along with NADPH and 5...
(Fig.4). Such a model has also been observed in other dietary interventions such as the effect of potassium43or sodium44intake on blood pressure or the DASH diet45. In such a model, even a small reduction in blood pressure can have a considerable impact on morbidity and mortality on a ...