The questionnaire used most frequently to evaluate sleep quality in both clinical practice and research is the Pittsburgh Sleep Quality Index (PSQI)7,8. Initially developed as an instrument to distinguish between good and poor sleep quality in the general population, it has subsequently been used in...
aPICOC analysis has been widely used in informing development of good research questions and will be adopted for this research. In highlighting the importance of PICO Analysis, Willson (2007) observes that this analysis plays an important role in research in as far as formulation of appropriate re...
The 5G12 primary antibody is specific for the EW(Man) motif found in the two TSR domains of MTRAP. Data is representative from n = 1 experiment. Full size image The 5G12 mAb was then used to confirm that C-mannosylation was occurring on MTRAP in P. falciparum blood stages. M...
The PICO (participants, interventions, comparators, and outcomes) criteria therefore included the following: participants were adults without cardiovascular disease (eg, the general population); interventions consisted of a CACS plus a CVD risk equation; comparators consisted of the same CVD risk ...
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RNA 6000 Pico Kit, for Bioanalyzer 2100 platform Agilent 5067-1513 Invitrogen™ Dynabeads™ Oligo(dT)25 mRNA isolation beads ThermoFisher 61005 Invitrogen™ RiboMinus™ Transcriptome Isolation Kit, human/mouse ThermoFisher K155002 NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® ...
aPICOC analysis has been widely used in informing development of good research questions and will be adopted for this research. In highlighting the importance of PICO Analysis, Willson (2007) observes that this analysis plays an important role in research in as far as formulation of appropriate re...
Three samples (GBM1–GBM3) were successfully used for a primary culture. Aborted fetuses were obtained from the Southwest Hospital for neuron cultures. Written informed consent was obtained from all subjects. The Institute Research Medical Ethics Committee of the Army Medical University granted ...
To determine the distribution of H3T3ph and H3K4me2/3 in mitosis using ChIP-seq, we required highly enriched populations of mitotic cells. We used HeLa cells because this is a well-characterized cell line that has been used for a number of comparable studies. We found that a double thymidi...
We developed the immunoblots using SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoScientific) per manufacturer’s protocol using a ChemiDox XRS imaging system (Bio-Rad). See Source Data for uncropped images. Signal was quantified with Image Studio Lite, and statistical differences were ...