Q.1 Is Phenol acidic or basic? Ans. Phenol is a poor acid. In aqueous solutions within the pH range of 8-12, it is in equilibrium with the phenolate anion C6H5O- (also phenoxide). Phenol is more acidic than aliphatic compounds as it contains an OH group. The phenoxide anion is st...
Why is phenol acidic? Phenol is acidic in naturebecause it can lose hydrogen ions from its OH bond, as on losing this hydrogen phenoxide ion is formed which is stable. Though it is a weak acid it is in equilibrium with the phenolate anion C6H5O− which is also called phenoxide. Why...
TRIzol® reagent is an acid-guanidinium-phenol based reagent ideally designed for the extraction of RNA (as well as DNA and protein) from various biological sample inputs. The low pH (acidic) of TRIzol® controls to separate RNA from DNA and protein, while a high pH can cause RNA and...
Acidic Tyrode’s solution (Sigma) containing 0.2% PVA was used to remove the zona pellucida (ZP) from embryos before sample collection. Then, 30 ZP-free morula-stage embryos and nucleofected ESCDuxs were lysed in Lysis Solution (from SuperPrep II Cell Lysis & RT Kit, TOYOBO), directly. ...
The medium was then removed and 0.2 ml/well of acidic isopropyl alcohol (0.04 M HCl in absolute isopropyl alcohol) was added. The absorbance of the converted dye was measured at 570 nm using a Synergy II spectrophotometer (Biotek). For colony formation assays, we generally plated ...
Soil organic matter stabilization in acidic forest soils is preferential and soil type-specific. Eur. J. Soil Sci. 59, 674e692.Spielvogel S, Prietzel J, Kogel-Knabner I (2008) Soil organic matter stabilization in acidic forest soils is preferential and soil type-specific. Eur J Soil Sci ...
Brønsted acidic ionic liquid [HMIm]BF4 has been used as a nonvolatile, ecofriendly solvent, and catalytic medium for the one-pot green synthesis of isoxazolyl-1,3-benzoxazines. The reaction afforded excellent yield in short reaction time, and avoids multistep synthesis. The easy of recyclabili...
Even 'flat' soft drinks are acidic. How can this be possible after all the carbonic acid has escaped? (Explain why this is expected.) Soft drink A soft drink is a beverage that typically has carbonated water, a sweetener, and either...
such as phenol, cresol, xylenol or other mono- or polyhydric phenol, or amino alcohols, such as dipropylaminoethanol, diethylaminopropanol, diethylaminoethanol, monoethanolamine, etc., to give esters of the types: CH2-CHCOOH / \ S NH CHr-HC0002CH Ca2-CHCOO0 (CH2),NRIRz / NR , e.g....
Influenza A viral HA0, the precursor of HA, is cleaved by cellular proteases like trypsin to produce HA1 and HA2, and this triggers the fusion of viral envelope and endosome membrane in an acidic environment (43., 44., 45.). We therefore tested HA digestion by trypsin and could not ...