Confirm PCR-positive results, after overnight incubation, using rapid chromogenic culture media methods. See how we have innovated to improve workflow & handling: Learn how you can reduce hands-on time whilst increasin...
This removes the need to quantify DNA products using time-consuming gel electrophoresis protocols. Applied Biosystems' TaqMan qPCR is based on the most important enzyme in PCR, Taq polymerase. With an average workflow time of just two hours, Applied Biosystems' TaqMan qPCR assays...
a,bHEK 293T cells expressing mouse LDLR, LDLRAD3 or TIMD4 or transfected with pCAGGS vector (negative control) were infected with the rGETV-EGFP. At 24 h post infection viral RNA (vRNA) levels in infected cells were measured via qRT-PCR (normalized relative to WT cells) (a) and v...
To compare their ability to tune endogenous gene expression, we targeted the repressor systems to the Esrrb gene in a mESC line, where the gene is homozygously tagged with P2A-mCherry (Fig. 2a)36. We then used flow cytometry to quantify repression at the single-cell level. Previous work ...
The efficiency (E) of PCR is defined as the fraction of target molecules that are copied in one PCR cycle [10], [11]. A properly designed assay shall, in the absence of interfering substances in the sample matrix, amplify target DNA with at least 90% efficiency [12], [13]. However,...
Upon formation of mono-clonal colonies, individual colonies were screened for the successful editing of the endogenous RPB1 (POLR2A) locus by genomic DNA extraction using Quick Extract kit (QE0905T), followed by PCR amplification of the corresponding gene regions. PCR reactions were cleaned up by...
Photorhabdus are motile members of the family Enterobactericeae that are pathogenic to insect larvae whilst also maintaining a mutualistic interaction with entomophagous nematodes of the family Heterorhabditiae. The interactions between Photorhabdus and its hosts are thought to be an obligate part of ...
Real-time PCR(often called qPCR) is usually conducted to quantify the absolute amount of a target sequence or to compare relative amounts of a target sequence between samples. This technique monitors the amplification of the target in real time via a target-specific fluorescent signal emitted duri...
a RT-PCR from liver and brain tissues obtained from the wild type and TMEM16K full knockout mice. Two different sets of primers amplifying TMEM16K were used, and Gadph and β-actin were amplified as controls. b Representative images and quantification of neuromuscular junction (NMJ) at 6 mon...
4F). When used to quantify virus in a plaque assay, the enhanced sensitivity of gFcRn (and gFcRn+gCD163) overexpressing cells resulted in detection of 5-10-fold more virus in the same sample (Fig. 4F, G). Fig. 4: FcRn overexpression enhances arterivirus infection. A Western blot ...