quantitative PCRrRNATaqManSummary Quantitative diagnostic PCR systems based upon rDNA targeted primer and probe combinations were developed for the detection of Escherichia coli, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas alcaligenes, enterococci, Staphylococcus aureus, and Staphylococcus epidermidis...
Real-time PCR results can either be qualitative (the presence or absence of a sequence) or quantitative (copy number). Quantitative real-time PCR is thus also known as qPCR analysis. In contrast, PCR is at best semiquantitative. Additionally, real-time qPCR data can be evaluated without gel...
6. The X method is one of the more practical ways of ... 7. It was considered that quantitative measures would usefully supplement and extend the qualitative analysis. 8. Many of the distributions were not normal so non-parametric signed rank tests were run. 9. The X approach has a numb...
We next used interval mapping to localize regions of the genome that influence variation in tail traits in our F2 hybrid population. For total tail length, we identified six significant quantitative trait loci (QTL) that, together in a multiple-QTL model, explain 23.8% of the variance in tail...
To better understand the dynamics of ookinete infection, mature gametocytes were fed to mosquitoes and quantitative PCR was performed to measure expression of parasite and mosquito genes the day after blood feeding. Expression of P. falciparum genes Pfs25 and CTRP was similar across conditions, indica...
Qualitative and Quantitative RT-PCR Isolation of total RNA, DNase I treatment, and reverse transcription were performed as described previously (16). For the qualitative RT-PCR analysis of the conjugation operon, 1.5 μl of the cDNA reactions, 1.5 μl of the cDNA reactions omitting reverse trans...
Systematic assessment of the reliability of quantitative PCR assays targeting IS900 for the detection of Mycobacterium avium ssp. paratuberculosis presence in ... N Bissonnette,JP Brousseau,S Ollier,... - 《Journal of Dairy Science》 被引量: 0发表: 2024年 Comparison of Real-Time, Quantitative ...
aAmong the different methods for detecting transgenic materials that are in use today, real-time quantitative PCR is the most powerful, accessible, and cost efficient (Higuchi et al. 1992). 在那今天是使用中的用于检测跨基因的材料的不同方法中,实时数量的 PCR 是最强大的,可接近,花费有效 ( Higuch...
evolving quantitative and qualitative neutralizing response to SARS-CoV-2 and VOCs in children less than 5 years old and who are currently ineligible for vaccination. This is especially important as children in this study were infected with SARS-CoV-2 prior to the circulation of Delta or ...
Cells were either lysed for quantitative PCR (qPCR) or plated at 2 × 105 cells per well in flat bottomed 96-well plates in RPMI (Invitrogen) containing 10% FCS, 2 mM l-glutamine, 0.05 mM 2-mercaptoethanol and 100 U ml−1 each of penicillin and streptomycin (complete ...